Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - A, Immunolocalisation of F-actin and vinculin in A431/SIP1 cells maintained with or without DOX for 48 hours. B, Adhesion assay of cells cultured with or without DOX for 48 hours. Cells were plated on collagen type I- or fibronectin-coated wells and the proportion of adhered cells was quantified in 15 min (see Materials and Methods). Results are mean ± SD of triplicate experiments.
• Supplemental Figure 2 - Validation of the cDNA microarray data. Expression of the selected genes in cells expressing wild-type SIP1 or SIP1 with the mutated C-terminal Zn-finger cluster was analysed by RT PCR.
• Supplemental Table 1A - cDNA microarray analysis of genes differentially regulated by SIP1. cDNA was prepared from A431/SIP1 cells cultured with or without DOX for 48 hours. A, Genes up-regulated by a factor of 1.8 or more. B, Genes down-regulated by a factor of 0.55 or less.
• Supplemental Table 1B