Supplemental Materials

This article contains the following supporting material:

• Figure S1 - (A) Immunofluorescence of microtubules, Eg5 and Kif2a from prophase to metaphase in LLC-Pk1-PA cells. (B) Deconvolved image of the bottom prophase cell from (A). Bar, 10µm.
• Figure S2 - Prophase centrosomes undergo slow motility (0.65µm/min) characterized by frequent pauses within the time frame of typical prophase photoactivation experiments (90sec). (A) A time-lapse sequence of an LLC-Pk1 cell expressing GFP-EB1. A phase contrast image acquired prior to the time-lapse is displayed as an inset. Blue and green circles serve as fiduciary marks against which centrosomal movement can be visualized. Time points are in min:sec. (B) For each centrosome in (A), the Pythagorean distance from the origin is plotted against time. The centrosome associated with each plot is coordinated with (A) by color. The inset displays the average percentage of time centrosomes are mobile and static (defined as at least three consecutive frames without detectable motion). Bar, 10µm.
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