Type I PIP Kinase Is a Novel Uropod Component that Regulates Rear Retraction during Neutrophil Chemotaxis

Supplemental Materials

This article contains the following supporting material:

• Supplemental Figure 1 - High levels of exogenously expressed PIPKIγ impairs chemotaxis of dHL-60 cells. (A) Representative immunoblot shows expression of GFP-PIPKIγ661 as compared to endogenous PIPKIγ expression in dHL-60 cells. dHL-60 cells were sorted into high (HI) and low (LO) expressing populations by flow cytometry. Quantitative analysis of GFP-PIPKIγ661 expression is shown. Fold overexpression was determined using densitometry as described in materials and methods. (B) Representative time-lapse sequence of dHL-60 cells that express high levels of GFP control. (C) Representative time-lapse sequence of dHL-60 cells that express high levels of wild-type (WT) GFP-PIPKIγ661. (D) Representative time-lapse sequence of dHL-60 cells that express high levels of kinase-dead (KD) GFP-PIPKIγ661. The direction of the chemoattractant source is denoted with a filled white circle (o). Scale bars, 5µm.
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