MBC Videos

This article contains the following supporting material:

• Figure S1 - ZEN-4 FORMS PARALLEL COILED COIL DIMERS
  Recombinant ZEN-4(1-556)C547V-Gly-Gly-Cys, ZEN-4(1-539)Gly-Gly-Cys and ZEN-4(1-603), which contains a cysteine residue at position 547, were incubated under oxidizing conditions. The reaction was quenched and the ability of ZEN-4 to form disulfide bridges was analyzed. ZEN-4(603) as well as ZEN-4(1-556)C547V-Gly-Gly-Cys efficiently formed dimers, whereas ZEN-4(1-539)Gly-Gly-Cys did not. Thus, ZEN-4 forms a parallel coiled coil dimer.
• Movie 01 - MODEL OF FUNCTIONAL AND STRUCTURAL ASPECTS OF THE CENTRALSPINDLIN COMPLEX
  Animation depicting the organization of centralspindlin and one model for how centralspindlin bundles antiparallel microtubules during cytokinesis. See text for details.