Proper Recruitment of -Tubulin and D-TACC/Msps to Embryonic Drosophila Centrosomes Requires Centrosomin Motif 1

Supplemental Materials

This article contains the following supporting material:

• Movie 1 - Centrosome dynamics in cnn^WT embryos.
  Live imaging of GFP-Cnn^WT centrosomes in a cnn null mutant background (cnn^WT embryos) through cleavage cycles 10-12. The still images in Figure 2A-F were taken from this movie. Note the concerted division of centrosomes at telophase and their separation in interphase. Also evident are the dynamic movement of satellite particles, which are severely decreased in cnn^Δ1 embryos (Movie 2). The time code is shown in seconds in the lower right corner. Images were captured at 3 sec intervals, and are displayed at 15 frames per second.
• Movie 2 - Centrosome separation and satellite trafficking defects in cnn^Δ1 embryos.
  Live imaging of GFP-Cnn^Δ1 centrosomes in a cnn null mutant background (cnn^Δ1 embryos) through cleavage cycles 10-13. The still images in Figure 2G-L were taken from this movie. In contrast to cnn^WT embryos, centrosomes fail to separate after duplication and are found predominantly in pairs or groups of pairs. Note the decrease in satellites compared to cnn^WT (Movie 1). The time code is shown in seconds in the lower right corner. Images were captured at 3 sec intervals, and are displayed at 15 frames per second.
• Table S1