Supplemental Material

This article contains the following supporting material:

• Supplementary Figure 1 - DNA content was measured by flow cytometry during the experiments shown in Figures 1-3.
• Supplementary Figure 2 - Pausing of DNA replication forks in the rDNA was analysed in the same experiments described in Figures 4 and 5. Pausing at the RFB requires Tof1 but not Mrc1, as observed previously.
• Supplementary Table 1 - Strains used in this study.
• Supplementary Table 2 - The rate of progression of the leftward fork from ARS306 in the indicated strains.