Supplemental Materials

This article contains the following supporting material:

• Movie 1 - Microtubule behavior in wild type (SNT22). Pictures taken every 3 sec in both channels for 87 sec (exposure times 200 msec). GFP-MTs elongated towards the hyphal tip and merged at the mRFP1-TeaA point.
• Movie 2 - Microtubule behavior in ΔteaA (SNT31). Pictures taken every 1.1 sec for 50 sec (exposure times 200 msec). In the ΔteaA mutant, GFP-MTs did not merge in one point at the apex.
• Movie 3 - Microtubule behavior in ΔteaA (SNT31). Pictures taken every 2 sec for 64 sec (exposure times 200 msec). In the ΔteaA mutant, GFP-MTs bended after they attached the cortex.
• Movie 4 - Dynamics of KipA and TeaA (SNT23). Pictures taken every 3 sec in both channels for a period of 30 sec (exposure times 300 msec). GFP-KipA at MT plus ends moved towards the hyphal tip and merged at one point at the apex with mRFP1-TeaA. mRFP1-TeaA cannot be detected at the growing MT plus ends.
• Movie 5 - Dynamics of TeaA in ΔkipA (SNT23). Pictures taken every 1 min for a period of 10 min (exposure times 200 msec). In the ΔkipA mutant, mRFP1-TeaA moved away from the center of the apex to the side of the tip and divided into two points.