Supplemental Materials

This article contains the following supporting material:

• Figure S1 - The relative phosphorylation intensity of Pan1p and Scd5p in various mutants. The phosphorylation level of each protein was measured by densitometer and normalized against its amount. The relative phosphorylation intensities were then calculated and presented as bar graphs. (A-B) The relative phosphorylation intensities of Pan1-Myc in Figure 3D (A), and Figure 3E (B). (C-E) The relative phosphorylation intensities of Scd5-HA in Figure 3H (C), Figure 3I (D), and Figure 3J (E).
• Movie 1 - Pan1-GFP dynamics on cortical patches. Interval between frames is 2 s.
• Movie 2 - Scd5-GFP dynamics on cortical patches. Interval between frames is 4 s.
• Movie 3 - Prk1-GFP dynamics on cortical patches. Interval between frames is 2 s.
• Movie 4 - Localization of Scd5-GFP (shown in green) in reference to Pan1-CFP (shown in red) on cortical patches. Interval between frames is 5 s.
• Movie 5 - Localization of Prk1-GFP (shown in green) in reference to Pan1-CFP (shown in red) on cortical patches. Interval between frames is 3 s.