Membrane-active Compounds Activate the Transcription Factors Pdr1 and Pdr3 Connecting Pleiotropic Drug Resistance and Membrane Lipid Homeostasis in Saccharomyces cerevisiae

Supplemental Materials

This article contains the following supporting material:

• Supplementary Figure S1 - Structures of substances reported to activate Pdr1 and/or Pdr3.
• Supplementary Figure S2 - Cluster analysis of DCP, POELE, 2,4-D and Fluphenazine response (A) Comparison of 167 2,4-D induced genes as reported by Teixeira et al., (Teixeira et al., 2006) with the corresponding values obtained with DCP and POELE. Genes with increased levels upon Msn2 overexpression (Chua et al., 2006) (Msn2-OE) and the fold difference of wild type to pdr1Δ pdr3Δ (Pdr1/Pdr3 dependency) were included in the visualization. The 2,4-D profile is similar to DCP and POELE for and includes Msn2/Msn4- and Pdr1/Pdr3-regulated gene clusters but lacks a detectable HSE and Crz1 response. (B) Fluphenazine, DCP and POELE responses overlap. The 20 minutes time point of wild type and pdr1Δ pdr3Δ mutant treated with Fluphenazine as reported (Fardeau et al., 2007) were compared to the 20-minute exposure values for DCP and POELE.
• Supplementary Figure S3 - Cluster analysis Ino4 and Rpn4 target genes of DCP and POELE treatment. Ino4 (A) and Rpn4 (B) target genes are induced significantly by DCP and POELE. Pdr1 and Pdr3 do not influence on the expression pattern of these genes.