![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on April 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on May 10, 2001
Revised on December 21, 2001
Accepted on December 31, 2001
in RBL-2H3 cells
1 Institute for Cancer Studies, Birmingham University, Birmingham, B15 2TA, United Kingdom
2 Institute for Cancer Studies, Birmingham University, Birmingham, B15 2TA, United Kingdom (present address: Biological Sciences, Warwick University, Coventry, CV4 7AL, United Kingdom)
* Corresponding author. E-mail address: m.j.o.wakelam{at}bham.ac.uk.
Phospholipase D (PLD) activity can be detected in response to many agonists in most cell types, however the pathway from receptor occupation to enzyme activation remains unclear. In vitro PLD1b activity is phosphatidylinositol 4,5-bisphosphate-dependent via an N-terminal PH domain and is stimulated by Rho, ARF and PKC family proteins, combinations of which cooperatively increase this activity. Here we provide the first evidence for the in vivo regulation of PLD1b at the molecular level. Antigen stimulation of RBL-2H3 cells induces the co-localisation of PLD1b with Rac1, ARF6 and PKC at the plasma membrane in actin-rich structures, simultaneously with cooperatively increasing PLD activity. Activation is both specific and direct since dominant negative mutants of Rac1 and ARF6 inhibit stimulated PLD activity and surface plasmon resonance reveals that the regulatory proteins bind directly and independently to PLD1b. This also indicates that PLD1b can concurrently interact with a member from each regulator family. Our results show that in contrast to PLD1b's translocation to the plasma membrane, PLD activation is phosphatidylinositol 3-kinase-dependent. Therefore as inactive, dominant negative GTPases do not activate PLD1b, we propose that activation results from phosphatidylinositol 3-kinase-dependent stimulation of Rac1, ARF6 and PKC.
This article has been cited by other articles:
|
|
 |
|
  F. A. Lisboa, Z. Peng, C. A. Combs, and M. A. Beaven Phospholipase D Promotes Lipid Microdomain-Associated Signaling Events in Mast Cells J. Immunol., October 15, 2009; 183(8): 5104 - 5112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. V. Usatyuk, I. A. Gorshkova, D. He, Y. Zhao, S. K. Kalari, J. G. N. Garcia, and V. Natarajan Phospholipase D-mediated Activation of IQGAP1 through Rac1 Regulates Hyperoxia-induced p47phox Translocation and Reactive Oxygen Species Generation in Lung Endothelial Cells J. Biol. Chem., May 29, 2009; 284(22): 15339 - 15352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Momboisse, E. Lonchamp, V. Calco, M. Ceridono, N. Vitale, M.-F. Bader, and S. Gasman {beta}PIX-activated Rac1 stimulates the activation of phospholipase D, which is associated with exocytosis in neuroendocrine cells J. Cell Sci., March 15, 2009; 122(6): 798 - 806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Chae, J. H. Kim, K. L. Kim, H. W. Kim, H. Y. Lee, W. D. Heo, T. Meyer, P.-G. Suh, and S. H. Ryu Phospholipase D Activity Regulates Integrin-mediated Cell Spreading and Migration by Inducing GTP-Rac Translocation to the Plasma Membrane Mol. Biol. Cell, July 1, 2008; 19(7): 3111 - 3123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gorshkova, D. He, E. Berdyshev, P. Usatuyk, M. Burns, S. Kalari, Y. Zhao, S. Pendyala, J. G. N. Garcia, N. J. Pyne, et al. Protein Kinase C-{epsilon} Regulates Sphingosine 1-Phosphate-mediated Migration of Human Lung Endothelial Cells through Activation of Phospholipase D2, Protein Kinase C-{zeta}, and Rac1 J. Biol. Chem., April 25, 2008; 283(17): 11794 - 11806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gomez-Cambronero, M. Di Fulvio, and K. Knapek Understanding phospholipase D (PLD) using leukocytes: PLD involvement in cell adhesion and chemotaxis J. Leukoc. Biol., August 1, 2007; 82(2): 272 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mazie, J. K. Spix, E. R. Block, H. B. Achebe, and J. K. Klarlund Epithelial cell motility is triggered by activation of the EGF receptor through phosphatidic acid signaling J. Cell Sci., April 15, 2006; 119(8): 1645 - 1654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Choi, Z. A. Karim, and S. W. Whiteheart Arf6 plays an early role in platelet activation by collagen and convulxin Blood, April 15, 2006; 107(8): 3145 - 3152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, H. Liao, A. Castle, J. Zhang, J. Casanova, G. Szabo, and D. Castle SCAMP2 Interacts with Arf6 and Phospholipase D1 and Links Their Function to Exocytotic Fusion Pore Formation in PC12 Cells Mol. Biol. Cell, October 1, 2005; 16(10): 4463 - 4472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Powner, R. M. Payne, T. R. Pettitt, M. L. Giudici, R. F. Irvine, and M. J. O. Wakelam Phospholipase D2 stimulates integrin-mediated adhesion via phosphatidylinositol 4-phosphate 5-kinase I{gamma}b J. Cell Sci., July 1, 2005; 118(13): 2975 - 2986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. I. Mead, Y. Zheng, C. N. Manzotti, L. C. A. Perry, M. K. P. Liu, F. Burke, D. J. Powner, M. J. O. Wakelam, and D. M. Sansom Exocytosis of CTLA-4 Is Dependent on Phospholipase D and ADP Ribosylation Factor-1 and Stimulated during Activation of Regulatory T Cells J. Immunol., April 15, 2005; 174(8): 4803 - 4811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Komati, F. Naro, S. Mebarek, V. De Arcangelis, S. Adamo, M. Lagarde, A.-F. Prigent, and G. Nemoz Phospholipase D Is Involved in Myogenic Differentiation through Remodeling of Actin Cytoskeleton Mol. Biol. Cell, March 1, 2005; 16(3): 1232 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hitomi, J. Zhang, L. M. Nicoletti, A. C. G. Grodzki, M. C. Jamur, C. Oliver, and R. P. Siraganian Phospholipase D1 regulates high-affinity IgE receptor-induced mast cell degranulation Blood, December 15, 2004; 104(13): 4122 - 4128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Cho, H.-J. You, C.-H. Woo, Y.-J. Yoo, and J.-H. Kim Rac and Protein Kinase C-{delta} Regulate ERKs and Cytosolic Phospholipase A2 in Fc{epsilon}RI Signaling to Cysteinyl Leukotriene Synthesis in Mast Cells J. Immunol., July 1, 2004; 173(1): 624 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Huang, K. M. Hujer, Z. Wu, and R. T. Miller The Ca2+-sensing receptor couples to G{alpha}12/13 to activate phospholipase D in Madin-Darby canine kidney cells Am J Physiol Cell Physiol, January 1, 2004; 286(1): C22 - C30. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jose Lopez-Andreo, J. C. Gomez-Fernandez, and S. Corbalan-Garcia The Simultaneous Production of Phosphatidic Acid and Diacylglycerol Is Essential for the Translocation of Protein Kinase C{epsilon} to the Plasma Membrane in RBL-2H3 Cells Mol. Biol. Cell, December 1, 2003; 14(12): 4885 - 4895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Donaldson Multiple Roles for Arf6: Sorting, Structuring, and Signaling at the Plasma Membrane J. Biol. Chem., October 24, 2003; 278(43): 41573 - 41576. [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Foster and L. Xu Phospholipase D in Cell Proliferation and Cancer Mol. Cancer Res., September 1, 2003; 1(11): 789 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gidwani, H. A. Brown, D. Holowka, and B. Baird Disruption of lipid order by short-chain ceramides correlates with inhibition of phospholipase D and downstream signaling by Fc{epsilon}RI J. Cell Sci., August 1, 2003; 116(15): 3177 - 3187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Xu, P. Frankel, D. Jackson, T. Rotunda, R. L. Boshans, C. D'Souza-Schorey, and D. A. Foster Elevated Phospholipase D Activity in H-Ras- but Not K-Ras-Transformed Cells by the Synergistic Action of RalA and ARF6 Mol. Cell. Biol., January 15, 2003; 23(2): 645 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. O'Luanaigh, R. Pardo, A. Fensome, V. Allen-Baume, D. Jones, M. R. Holt, and S. Cockcroft Continual Production of Phosphatidic Acid by Phospholipase D Is Essential for Antigen-stimulated Membrane Ruffling in Cultured Mast Cells Mol. Biol. Cell, October 1, 2002; 13(10): 3730 - 3746. [Abstract] [Full Text] [PDF]
|
|
