![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on October 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on December 4, 2001
Revised on May 29, 2002
Accepted on July 16, 2002
1 Junior Research Group of Molecular Cell Biology, Institute of Environmental Health Research, Heinrich-Heine-University, Düsseldorf, Germany
2 Department of Molecular Biology, Institute of Molecular Biotechnology, Jena, Germany
3 Computing Center, Technical University, Aachen, Germany
* Corresponding author. E-mail address: mikecz{at}uni-duesseldorf.de.
A prerequisite for proteins to interact in a cell is that they are present in the same intracellular compartment. Although it is generally accepted that proteasomes occur in both, the cytoplasm and the nucleus, research has been focussing on cytoplasmic protein breakdown and antigen processing, respectively. Thus, little is known on the functional organization of the proteasome in the nucleus. Here we report that within the nucleus 20S and 26S proteasomes occur throughout the nucleoplasm, and partially colocalize with splicing factor-containing speckles. Since proteasomes are absent from the nucleolus, a recruitment system was used to analyse the molecular fate of nucleolar protein fibrillarin: Subtoxic concentrations of mercuric chloride (HgCl2) induce subcellular redistribution of fibrillarin and substantial colocalization (33%) with nucleoplasmic proteasomes in different cell lines, and in primary cells isolated from mercury-treated mice. Accumulation of fibrillarin and fibrillarin-ubiquitin conjugates in lactacystin-treated cells suggests that proteasome-dependent processing of this autoantigen occurs upon mercury induction. The latter observation might constitute the cell biological basis of autoimmune responses that specifically target fibrillarin in mercury-mouse models and scleroderma.
This article has been cited by other articles:
|
|
 |
|
  M. Simoneau, J. Boulanger, G. Coulombe, M.-A. Renaud, C. Duchesne, and N. Rivard Activation of Cdk2 Stimulates Proteasome-dependent Truncation of Tyrosine Phosphatase SHP-1 in Human Proliferating Intestinal Epithelial Cells J. Biol. Chem., September 12, 2008; 283(37): 25544 - 25556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Baldin, M. Militello, Y. Thomas, C. Doucet, W. Fic, S. Boireau, I. Jariel-Encontre, M. Piechaczyk, E. Bertrand, J. Tazi, et al. A Novel Role for PA28{gamma}-Proteasome in Nuclear Speckle Organization and SR Protein Trafficking Mol. Biol. Cell, April 1, 2008; 19(4): 1706 - 1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Y. Rawe, E. S. Diaz, R. Abdelmassih, C. Wojcik, P. Morales, P. Sutovsky, and H. E. Chemes The role of sperm proteasomes during sperm aster formation and early zygote development: implications for fertilization failure in humans Hum. Reprod., March 1, 2008; 23(3): 573 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Philips, J. C. Kwok, and B. H. Chong Analysis of the Signals and Mechanisms Mediating Nuclear Trafficking of GATA-4: LOSS OF DNA BINDING IS ASSOCIATED WITH LOCALIZATION IN INTRANUCLEAR SPECKLES J. Biol. Chem., August 24, 2007; 282(34): 24915 - 24927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Stavreva, M. Kawasaki, M. Dundr, K. Koberna, W. G. Muller, T. Tsujimura-Takahashi, W. Komatsu, T. Hayano, T. Isobe, I. Raska, et al. Potential Roles for Ubiquitin and the Proteasome during Ribosome Biogenesis. Mol. Cell. Biol., July 1, 2006; 26(13): 5131 - 5145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. von Mikecz The nuclear ubiquitin-proteasome system J. Cell Sci., May 15, 2006; 119(10): 1977 - 1984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Rockel, D. Stuhlmann, and A. von Mikecz Proteasomes degrade proteins in focal subdomains of the human cell nucleus J. Cell Sci., November 15, 2005; 118(22): 5231 - 5242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Sutovsky, G. Manandhar, T. C. McCauley, J. N. Caamano, M. Sutovsky, W. E. Thompson, and B. N. Day Proteasomal Interference Prevents Zona Pellucida Penetration and Fertilization in Mammals Biol Reprod, November 1, 2004; 71(5): 1625 - 1637. [Abstract] [Full Text] [PDF]
|
|
