![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on February 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 3, 2002
Accepted on October 21, 2002
1 Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Sakamoto 9, Asamushi, Aomori, Aomori 039-3501, Japan
2 National Institute for Basic Biology, Okazaki 444-8585, Japan (present address: Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030-3305)
3 National Institute for Basic Biology, Okazaki 444-8585, Japan; and Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan
4 Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Sakamoto 9, Asamushi, Aomori, Aomori 039-3501, Japan; and National Institute for Basic Biology, Okazaki 444-8585, Japan
* Corresponding author. E-mail address: inaba{at}biology.tohoku.ac.jp.
Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidian Ciona intestinalis using an antiserum against whole axonemes. We report here a 37 kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22+ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported in Chlamydomonas and sea urchin flagella, and thus represents a novel axonemal LRR protein. Immunoelectron microscopy using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact appears to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.
This article has been cited by other articles:
|
|
 |
|
  E. van Rooijen, R. H. Giles, E. E. Voest, C. van Rooijen, S. Schulte-Merker, and F. J. van Eeden LRRC50, a Conserved Ciliary Protein Implicated in Polycystic Kidney Disease J. Am. Soc. Nephrol., June 1, 2008; 19(6): 1128 - 1138. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Yang, H. A. Owen, and P. Yang Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella J. Cell Biol., January 28, 2008; 180(2): 403 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Gaillard, L. A. Fox, J. M. Rhea, B. Craige, and W. S. Sale Disruption of the A-Kinase Anchoring Domain in Flagellar Radial Spoke Protein 3 Results in Unregulated Axonemal cAMP-dependent Protein Kinase Activity and Abnormal Flagellar Motility Mol. Biol. Cell, June 1, 2006; 17(6): 2626 - 2635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nishimune and H. Tanaka Infertility Caused by Polymorphisms or Mutations in Spermatogenesis-Specific Genes J Androl, May 1, 2006; 27(3): 326 - 334. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hui, J. Lu, Y. Han, and S. H. Pilder The Mouse t Complex Gene Tsga2, Encoding Polypeptides Located in the Sperm Tail and Anterior Acrosome, Maps to a Locus Associated with Sperm Motility and Sperm-Egg Interaction Abnormalities Biol Reprod, April 1, 2006; 74(4): 633 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Yang, D. R. Diener, C. Yang, T. Kohno, G. J. Pazour, J. M. Dienes, N. S. Agrin, S. M. King, W. S. Sale, R. Kamiya, et al. Radial spoke proteins of Chlamydomonas flagella J. Cell Sci., March 15, 2006; 119(6): 1165 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yang, M. M. Compton, and P. Yang Dimeric Novel HSP40 Is Incorporated into the Radial Spoke Complex during the Assembly Process in Flagella Mol. Biol. Cell, February 1, 2005; 16(2): 637 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Yang, C. Yang, and W. S. Sale Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii Eukaryot. Cell, February 1, 2004; 3(1): 72 - 81. [Abstract] [Full Text] [PDF]
|
|
