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Vol. 13, Issue 4, 1109-1121, April 2002
Departament de Ciències Mèdiques Bàsiques,
Facultat de Medicina, Universitat de Lleida, 25198-Lleida, Spain
Yeast cells contain a family of three monothiol glutaredoxins:
Grx3, 4, and 5. Absence of Grx5 leads to constitutive oxidative damage,
exacerbating that caused by external oxidants. Phenotypic defects
associated with the absence of Grx5 are suppressed by overexpression of
SSQ1 and ISA2, two genes involved in the
synthesis and assembly of iron/sulfur clusters into proteins. Grx5
localizes at the mitochondrial matrix, like other proteins involved in
the synthesis of these clusters, and the mature form lacks the first 29 amino acids of the translation product. Absence of Grx5 causes: 1) iron
accumulation in the cell, which in turn could promote oxidative damage,
and 2) inactivation of enzymes requiring iron/sulfur clusters for their
activity. Reduction of iron levels in grx5 null mutants
does not restore the activity of iron/sulfur enzymes, and cell growth
defects are not suppressed in anaerobiosis or in the presence of
disulfide reductants. Hence, Grx5 forms part of the mitochondrial
machinery involved in the synthesis and assembly of iron/sulfur centers.
Corresponding author. E-mail address:
enric.herrero{at}cmb.udl.es.
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