![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 4, 1158-1174, April 2002
Department of Molecular Genetics and Microbiology, University of
Massachusetts Medical School, Worcester Massachusetts 01655
Yeast protein insertion orientation (PIO) mutants
were isolated by selecting for growth on sucrose in cells in which the
only source of invertase is a C-terminal fusion to a
transmembrane protein. Only the fraction with an exocellular C
terminus can be processed to secreted invertase and this fraction is
constrained to 2-3% by a strong charge difference signal. Identified
pio mutants increased this to 9-12%.
PIO1 is SPF1, encoding a P-type ATPase located in the endoplasmic reticulum (ER) or Golgi.
spf1-null mutants are modestly sensitive to EGTA.
Sensitivity is considerably greater in an spf1 pmr1
double mutant, although PIO is not further disturbed. Pmr1p is the
Golgi Ca2+ ATPase and Spf1p may be the equivalent ER pump.
PIO2 is STE24, a metalloprotease anchored
in the ER membrane. Like Spf1p, Ste24p is expressed in all yeast cell
types and belongs to a highly conserved protein family. The effects of
ste24- and spf1-null mutations on
invertase secretion are additive, cell generation time is increased 60%, and cells become sensitive to cold and to heat shock. Ste24p and
Rce1p cleave the C-AAX bond of farnesylated CAAX box proteins. The
closest paralog of SPF1 is YOR291w.
Neither rce1-null nor yor291w-null
mutations affected PIO or the phenotype of spf1- or
ste24-null mutants. Mutations in PIO3
(unidentified) cause a weaker Pio phenotype, enhanced by a null
mutation in BMH1, one of two yeast 14-3-3 proteins.
Present address: Department of Developmental and
Molecular biology, Albert Einstein College of Medicine, Bronx, NY 10461.
This article has been cited by other articles:
|
|
 |
|
  S. Pu, K. Ronen, J. Vlasblom, J. Greenblatt, and S. J. Wodak Local coherence in genetic interaction patterns reveals prevalent functional versatility Bioinformatics, October 15, 2008; 24(20): 2376 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lockshon, L. E. Surface, E. O. Kerr, M. Kaeberlein, and B. K. Kennedy The Sensitivity of Yeast Mutants to Oleic Acid Implicates the Peroxisome and Other Processes in Membrane Function Genetics, January 1, 2007; 175(1): 77 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Jakobsen,, L. R. Poulsen,, A. Schulz,, P. Fleurat-Lessard,, A. Moller,, S. Husted,, M. Schiott,, A. Amtmann,, and M. G. Palmgren, Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a Type V P-type ATPase Genes & Dev., November 15, 2005; 19(22): 2757 - 2769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. N. Alder and A. E. Johnson Cotranslational Membrane Protein Biogenesis at the Endoplasmic Reticulum J. Biol. Chem., May 28, 2004; 279(22): 22787 - 22790. [Full Text] [PDF]
|
|
|
|
 |
|
 V. Goder, T. Junne, and M. Spiess Sec61p Contributes to Signal Sequence Orientation According to the Positive-Inside Rule Mol. Biol. Cell, March 1, 2004; 15(3): 1470 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vashist, C. G. Frank, C. A. Jakob, and D. T.W. Ng Two Distinctly Localized P-Type ATPases Collaborate to Maintain Organelle Homeostasis Required for Glycoprotein Processing and Quality Control Mol. Biol. Cell, November 1, 2002; 13(11): 3955 - 3966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Bergo, B. Gavino, J. Ross, W. K. Schmidt, C. Hong, L. V. Kendall, A. Mohr, M. Meta, H. Genant, Y. Jiang, et al. Zmpste24 deficiency in mice causes spontaneous bone fractures, muscle weakness, and a prelamin A processing defect PNAS, October 1, 2002; 99(20): 13049 - 13054. [Abstract] [Full Text] [PDF]
|
|
