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Vol. 14, Issue 11, 4641-4653, November 2003
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Laboratoire de Morphogenèse et Signalisation Cellulaires, Institut Curie Unité Mixte Recherche 144, 75248 Paris, France
Submitted February 19, 2003;
Revised June 23, 2003;
Accepted June 25, 2003
Monitoring Editor: Keith Mostov
Villin is an actin-binding protein localized to intestinal and kidney brush borders. In vitro, villin has been demonstrated to bundle and sever F-actin in a calcium-dependent manner. Although villin is not necessary for the bundling of F-actin in vivo, it is important for the reorganization of the actin cytoskeleton elicited by stress during both physiological and pathological conditions (Ferrary et al., 1999). These data suggest that villin may be involved in actin cytoskeleton remodeling necessary for many processes requiring cellular plasticity. Here, we study the role of villin in hepatocyte growth factor (HGF)-induced epithelial cell motility and morphogenesis. For this purpose, we used primary cultures of enterocytes derived from wild-type and villin knock-out mice and Madin-Darby canine kidney cells, expressing villin in an inducible manner. In vitro, we show that epithelial cell lysates from villin-expressing cells induced dramatic, calcium-dependent severing of actin filaments. In cell culture, we found that villin-expressing cells exhibit enhanced cell motility and morphogenesis upon HGF stimulation. In addition, we show that the ability of villin to potentiate HGF-induced actin reorganization occurs through the HGF-activated phospholipase C
signaling pathway. Collectively, these data demonstrate that villin acts as a regulator of HGF-induced actin dynamics.
, phospholipase C. * Corresponding author. E-mail address: sylvie.robine{at}curie.fr.
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