![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 14, Issue 3, 1074-1084, March 2003
*Department of Cell Biology and Oncology, Istituto di
Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro (Chieti), 66030 Italy; The degradation of extracellular matrix (ECM) by matrix
metalloproteases is crucial in physiological and pathological
cell invasion alike. Degradation occurs at specific sites where
invasive cells make contact with the ECM via specialized plasma
membrane protrusions termed invadopodia. Herein, we show that the
dynamin 2 (Dyn2), a GTPase implicated in the control of actin-driven
cytoskeletal remodeling events and membrane transport, is necessary for
focalized matrix degradation at invadopodia. Dynamin was inhibited by
using two approaches: 1) expression of dominant negative
GTPase-impaired or proline-rich domain-deleted Dyn2 mutants; and 2)
inhibition of the dynamin regulator calcineurin by cyclosporin A. In
both cases, the number and extension of ECM degradation foci were
drastically reduced. To understand the site and mechanism of dynamin
action, the cellular structures devoted to ECM degradation were
analyzed by correlative confocal light-electron microscopy. Invadopodia were found to be organized into a previously undescribed
ECM-degradation structure consisting of a large invagination of the
ventral plasma membrane surface in close spatial relationship with the
Golgi complex. Dyn2 seemed to be concentrated at invadopodia.
Endocrine
Unit, Department of Internal Medicine, Ospedale Civile "Renzetti",
Lanciano (Chieti), 66034 Italy; and Department
of Biochemistry and Molecular Biology, Center for Basic Research in
Digestive Diseases, Mayo Clinic, Rochester, Minnesota 55905
Online version of this article contains video material for some
figures. Online version is available at www.molbiolcell.org.
This article has been cited by other articles:
|
|
 |
|
  D. J. Sugarbaker, W. G. Richards, G. J. Gordon, L. Dong, A. De Rienzo, G. Maulik, J. N. Glickman, L. R. Chirieac, M.-L. Hartman, B. E. Taillon, et al. Transcriptome sequencing of malignant pleural mesothelioma tumors PNAS, March 4, 2008; 105(9): 3521 - 3526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ayala, M. Baldassarre, G. Giacchetti, G. Caldieri, S. Tete, A. Luini, and R. Buccione Multiple regulatory inputs converge on cortactin to control invadopodia biogenesis and extracellular matrix degradation J. Cell Sci., February 1, 2008; 121(3): 369 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Badowski, G. Pawlak, A. Grichine, A. Chabadel, C. Oddou, P. Jurdic, M. Pfaff, C. Albiges-Rizo, and M. R. Block Paxillin Phosphorylation Controls Invadopodia/Podosomes Spatiotemporal Organization Mol. Biol. Cell, February 1, 2008; 19(2): 633 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. C. Chan, P. D. Arora, and C. A. McCulloch Cyclosporin inhibition of collagen remodeling is mediated by gelsolin Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1049 - C1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Furmaniak-Kazmierczak, S. W. Crawley, R. L. Carter, D. H. Maurice, and G. P. Cote Formation of Extracellular Matrix-Digesting Invadopodia by Primary Aortic Smooth Muscle Cells Circ. Res., May 11, 2007; 100(9): 1328 - 1336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Clark, A. S. Whigham, W. G. Yarbrough, and A. M. Weaver Cortactin Is an Essential Regulator of Matrix Metalloproteinase Secretion and Extracellular Matrix Degradation in Invadopodia Cancer Res., May 1, 2007; 67(9): 4227 - 4235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Rothschild, A. H. Shim, A. G. Ammer, L. C. Kelley, K. B. Irby, J. A. Head, L. Chen, M. Varella-Garcia, P. G. Sacks, B. Frederick, et al. Cortactin Overexpression Regulates Actin-Related Protein 2/3 Complex Activity, Motility, and Invasion in Carcinomas with Chromosome 11q13 Amplification Cancer Res., August 15, 2006; 66(16): 8017 - 8025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Kruchten and M. A. McNiven Dynamin as a mover and pincher during cell migration and invasion. J. Cell Sci., May 1, 2006; 119(Pt 9): 1683 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Artym, Y. Zhang, F. Seillier-Moiseiwitsch, K. M. Yamada, and S. C. Mueller Dynamic interactions of cortactin and membrane type 1 matrix metalloproteinase at invadopodia: defining the stages of invadopodia formation and function. Cancer Res., March 15, 2006; 66(6): 3034 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Burgstaller and M. Gimona Podosome-mediated matrix resorption and cell motility in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H3001 - H3005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamaguchi, M. Lorenz, S. Kempiak, C. Sarmiento, S. Coniglio, M. Symons, J. Segall, R. Eddy, H. Miki, T. Takenawa, et al. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin J. Cell Biol., January 31, 2005; 168(3): 441 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mazzone, M. Baldassarre, G. Beznoussenko, G. Giacchetti, J. Cao, S. Zucker, A. Luini, and R. Buccione Intracellular processing and activation of membrane type 1 matrix metalloprotease depends on its partitioning into lipid domains J. Cell Sci., December 15, 2004; 117(26): 6275 - 6287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. McHugh, S. A. Krause, B. Yu, A.-M. Deans, S. Heasman, P. McLaughlin, and M. M.S. Heck Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration J. Cell Biol., November 22, 2004; 167(4): 673 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sanchez-San Martin, T. Lopez, C. F. Arias, and S. Lopez Characterization of Rotavirus Cell Entry J. Virol., March 1, 2004; 78(5): 2310 - 2318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schlunck, H. Damke, W. B. Kiosses, N. Rusk, M. H. Symons, C. M. Waterman-Storer, S. L. Schmid, and M. A. Schwartz Modulation of Rac Localization and Function by Dynamin Mol. Biol. Cell, January 1, 2004; 15(1): 256 - 267. [Abstract] [Full Text] [PDF]
|
|
