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Vol. 14, Issue 3, 916-925, March 2003
Department of Biological Sciences, Lehigh University,
Bethlehem, Pennsylvania 18015
A stable cell line expressing EB1-green fluorescent protein
was used to image growing microtubule plus ends at the G2/M
transition. By late prophase growing ends no longer extend to the cell
periphery and were not uniformly distributed around each centrosome.
Growing ends were much more abundant in the area surrounding the
nuclear envelope, and microtubules growing around the nucleus were 1.5 fold longer than those growing in the opposite direction. The growth of
longer ends toward the nucleus did not result from a localized faster
growth rate, because this rate was ~11 µm/min in all directions
from the centrosome. Rather, microtubule ends growing toward the
nucleus seemed stabilized by dynein/dynactin associated with the
nuclear envelope. Injection of p50 into late prophase cells removed
dynein from the nuclear envelope, reduced the density of growing ends
near the nuclear envelope and resulted in a uniform distribution of
growing ends from each centrosome. We suggest that the cell
cycle-dependent binding of dynein/dynactin to the nuclear envelope
locally stabilizes growing microtubules. Both dynein and microtubules
would then be in a position to participate in nuclear envelope
breakdown, as described in recent studies.
Online version of this article contains video material for some
figures. Online version available at www.molbiolcell.org.
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