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Vol. 14, Issue 4, 1717-1726, April 2003
The Pennsylvania State University, Department of Biology,
208 Mueller Laboratory, University Park, Pennsylvania 16801
Motor proteins have been implicated in various aspects of mitosis,
including spindle assembly and chromosome segregation. Here, we show
that acentrosomal Arabidopsis cells that are mutant for
the kinesin, ATK1, lack microtubule accumulation at the predicted spindle poles during prophase and have reduced spindle bipolarity during prometaphase. Nonetheless, all abnormalities are rectified by
anaphase and chromosome segregation appears normal. We conclude that
ATK1 is required for normal microtubule accumulation at the spindle
poles during prophase and possibly functions in spindle assembly during
prometaphase. Because aberrant spindle morphology in these mutants is
resolved by anaphase, we postulate that mitotic plant cells contain an
error-correcting mechanism. Moreover, ATK1 function seems to be
dosage-dependent, because cells containing one wild-type allele take
significantly longer to proceed to anaphase as compared with cells
containing two wild-type alleles.
Corresponding author. E-mail address:
rjc8{at}psu.edu.
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