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Vol. 15, Issue 5, 2287-2301, May 2004
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-tubulin Complex Function
* HHMI, Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232;
Departments Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
Submitted October 10, 2003;
Revised February 23, 2004;
Accepted February 23, 2004
Monitoring Editor: Trisha Davis
The 
-tubulin complex, via its ability to organize microtubules, is critical for accurate chromosome segregation and cytokinesis in the fission yeast, Schizosaccharomyces pombe. To better understand its roles, we have purified the S. pombe -tubulin complex. Mass spectrometric analyses of the purified complex revealed known components and identified two novel proteins (i.e., Mbo1p and Gfh1p) with homology to -tubulin–associated proteins from other organisms. We show that both Mbo1p and Gfh1p localize to microtubule organizing centers. Although cells deleted for either mbo1+ or gfh1+ are viable, they exhibit a number of defects associated with altered microtubule function such as defects in cell polarity, nuclear positioning, spindle orientation, and cleavage site specification. In addition, mbo1
and gfh1 cells exhibit defects in astral microtubule formation and anchoring, suggesting that these proteins have specific roles in astral microtubule function. This study expands the known roles of -tubulin complex components in organizing different types of microtubule structures in S. pombe.
Abbreviations used: EMTOC, equatorial microtubule organizing center; MTOC, microtubule organizing center; PAA, postanaphase array; SPB, spindle pole body.
Online version of this article contains supporting material.
Online version is available at www.molbiolcell.org.
Corresponding author. E-mail address: kathy.gould{at}vanderbilt.edu.
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