QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

Vol. 15, Issue 9, 4203-4214, September 2004

This Article Full Text Full Text (PDF) All Versions of this Article: E04-05-0420v1 15/9/4203    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bugnicourt, A. Articles by Galan, J.-M. Search for Related Content PubMed PubMed Citation Articles by Bugnicourt, A. Articles by Galan, J.-M.

Antagonistic Roles of ESCRT and Vps Class C/HOPS Complexes in the Recycling of Yeast Membrane Proteins

Amandine Bugnicourt ^*, Marine Froissard ^*, Kostianna Sereti ^*, Helle D. Ulrich , Rosine Haguenauer-Tsapis ^*, and Jean-Marc Galan ^* 

^* Institut Jacques Monod-Centre National de la Recherche Scientifique/Universités Paris 6 & 7, 75251 Paris Cedex 05, France; Max Planck Institute for Terrestrial Microbiology, D-35043 Marburg, Germany

Submitted May 19, 2004; Accepted June 14, 2004
Monitoring Editor: Howard Riezman

In Saccharomyces cerevisiae, deficiencies in the ESCRT machinery trigger the mistargeting of endocytic and biosynthetic ubiquitinated cargoes to the limiting membrane of the vacuole. Surprisingly, impairment of this machinery also leads to the accumulation of various receptors and transporters at the plasma membrane in both yeast and higher eukaryotes. Using the well-characterized yeast endocytic cargo uracil permease (Fur4p), we show here that the apparent stabilization of the permease at the plasma membrane in ESCRT mutants results from an efficient recycling of the protein. Whereas several proteins as well as internalized dyes are known to be recycled in yeast, little is known about the machinery and molecular mechanisms involved. The SNARE protein Snc1p is the only cargo for which the recycling pathway is well characterized. Unlike Snc1p, endocytosed Fur4p did not pass through the Golgi apparatus en route to the plasma membrane. Although ubiquitination of Fur4p is required for its internalization, deubiquitination is not required for its recycling. In an attempt to identify actors in this new recycling pathway, we found an unexpected phenotype associated with loss of function of the Vps class C complex: cells defective for this complex are impaired for recycling of Fur4p, Snc1p, and the lipophilic dye FM4-64. Genetic analyses indicated that these phenotypes were due to the functioning of the Vps class C complex in trafficking both to and from the late endosomal compartment.

Corresponding author. E-mail address: galan{at}ijm.jussieu.fr.

This article has been cited by other articles:

				T. I. Strochlic, B. C. Schmiedekamp, J. Lee, D. J. Katzmann, and C. G. Burd Opposing Activities of the Snx3-Retromer Complex and ESCRT Proteins Mediate Regulated Cargo Sorting at a Common Endosome Mol. Biol. Cell, November 1, 2008; 19(11): 4694 - 4706. [Abstract] [Full Text] [PDF]

				G. I. Miura, J.-Y. Roignant, M. Wassef, and J. E. Treisman Myopic acts in the endocytic pathway to enhance signaling by the Drosophila EGF receptor Development, June 1, 2008; 135(11): 1913 - 1922. [Abstract] [Full Text] [PDF]

				S. Leon, Z. Erpapazoglou, and R. Haguenauer-Tsapis Ear1p and Ssh4p Are New Adaptors of the Ubiquitin Ligase Rsp5p for Cargo Ubiquitylation and Sorting at Multivesicular Bodies Mol. Biol. Cell, June 1, 2008; 19(6): 2379 - 2388. [Abstract] [Full Text] [PDF]

				J. Kota, M. Melin-Larsson, P. O. Ljungdahl, and H. Forsberg Ssh4, Rcr2 and Rcr1 Affect Plasma Membrane Transporter Activity in Saccharomyces cerevisiae Genetics, April 1, 2007; 175(4): 1681 - 1694. [Abstract] [Full Text] [PDF]

				R. Kama, M. Robinson, and J. E. Gerst Btn2, a Hook1 Ortholog and Potential Batten Disease-Related Protein, Mediates Late Endosome-Golgi Protein Sorting in Yeast Mol. Cell. Biol., January 15, 2007; 27(2): 605 - 621. [Abstract] [Full Text] [PDF]

				G. Gabriely, R. Kama, and J. E. Gerst Involvement of Specific COPI Subunits in Protein Sorting from the Late Endosome to the Vacuole in Yeast Mol. Cell. Biol., January 15, 2007; 27(2): 526 - 540. [Abstract] [Full Text] [PDF]

				J. Ren, Y. Kee, J. M. Huibregtse, and R. C. Piper Hse1, a Component of the Yeast Hrs-STAM Ubiquitin-sorting Complex, Associates with Ubiquitin Peptidases and a Ligase to Control Sorting Efficiency into Multivesicular Bodies Mol. Biol. Cell, January 1, 2007; 18(1): 324 - 335. [Abstract] [Full Text] [PDF]

				G. S. Mourad, B. M. Snook, J. T. Prabhakar, T. A. Mansfield, and N. P. Schultes A fluoroorotic acid-resistant mutant of Arabidopsis defective in the uptake of uracil J. Exp. Bot., November 1, 2006; 57(14): 3563 - 3573. [Abstract] [Full Text] [PDF]

				M. Rubio-Texeira and C. A. Kaiser Amino Acids Regulate Retrieval of the Yeast General Amino Acid Permease from the Vacuolar Targeting Pathway Mol. Biol. Cell, July 1, 2006; 17(7): 3031 - 3050. [Abstract] [Full Text] [PDF]

				G. Wang and R. J. Deschenes Plasma Membrane Localization of Ras Requires Class C Vps Proteins and Functional Mitochondria in Saccharomyces cerevisiae Mol. Cell. Biol., April 15, 2006; 26(8): 3243 - 3255. [Abstract] [Full Text] [PDF]

				P. Gissen, C. A. Johnson, D. Gentle, L. D. Hurst, A. J. Doherty, C. J. O'Kane, D. A. Kelly, and E. R. Maher Comparative evolutionary analysis of VPS33 homologues: genetic and functional insights Hum. Mol. Genet., May 15, 2005; 14(10): 1261 - 1270. [Abstract] [Full Text] [PDF]