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Originally published as MBC in Press, 10.1091/mbc.E04-09-0765 on January 5, 2005

Vol. 16, Issue 3, 1178-1188, March 2005

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Three-dimensional Ultrastructure of Saccharomyces cerevisiae Meiotic Spindles{boxd}{boxv}

Mark Winey *, Garry P. Morgan *, Paul D. Straight * {dagger}, Thomas H. Giddings, Jr. *, and David N. Mastronarde * {ddagger}

* Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309-0347; {ddagger} Boulder Laboratory for 3-D Fine Structure, University of Colorado, Boulder, Boulder, CO 80309-0347

Submitted September 2, 2004; Revised December 10, 2004; Accepted December 21, 2004
Monitoring Editor: Ted Salmon

Meiotic chromosome segregation leads to the production of haploid germ cells. During meiosis I (MI), the paired homologous chromosomes are separated. Meiosis II (MII) segregation leads to the separation of paired sister chromatids. In the budding yeast Saccharomyces cerevisiae, both of these divisions take place in a single nucleus, giving rise to the four-spored ascus. We have modeled the microtubules in 20 MI and 15 MII spindles by using reconstruction from electron micrographs of serially sectioned meiotic cells. Meiotic spindles contain more microtubules than their mitotic counterparts, with the highest number in MI spindles. It is possible to differentiate between MI versus MII spindles based on microtubule numbers and organization. Similar to mitotic spindles, kinetochores in either MI or MII are attached by a single microtubule. The models indicate that the kinetochores of paired homologous chromosomes in MI or sister chromatids in MII are separated at metaphase, similar to mitotic cells. Examination of both MI and MII spindles reveals that anaphase A likely occurs in addition to anaphase B and that these movements are concurrent. This analysis offers a structural basis for considering meiotic segregation in yeast and for the analysis of mutants defective in this process.

This article was published online ahead of print in MBC in Press (http://www.molbiolcell.org/cgi/doi/10.1091/mbc.E04-09-0765) on January 5, 2005.

Abbreviations used: 3-D, three-dimensional; EM, electron microscopy; MI, meiosis I; MII, meiosis II; SPB, spindle pole body.

{boxd}{boxv} The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).

{dagger} Present address: Department of Microbiology, Harvard Medical School, Armenise D-219, 200 Longwood Ave., Boston, MA 02115.

Address correspondence to: Mark Winey (Mark.Winey{at}Colorado.edu).




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