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Vol. 18, Issue 2, 369-379, February 2007
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*Unité Mixte de Recherche Centre National de la Recherche Scientifique 5096, Université de Perpignan, Perpignan, 66860 Perpignan, France; Centro de Investigaciones Biológicas-Consejo Superior de Investigaciones Cientificas, Ramiro de Maetzu 9, E-28040 Madrid, Spain; and Unité Mixte de Recherche Centre National de la Recherche Scientifique 6547, BIOMOVE, Université Blaise Pascal, 63177 Aubière, France
Submitted August 25, 2006;
Revised October 27, 2006;
Accepted November 2, 2006
Monitoring Editor: Wendy Bickmore
Nucleolin is one of the most abundant protein in the nucleolus and is a multifunctional protein involved in different steps of ribosome biogenesis. In contrast to animals and yeast, the genome of the model plant Arabidopsis thaliana encodes two nucleolin-like proteins, AtNUC-L1 and AtNUC-L2. However, only the AtNUC-L1 gene is ubiquitously expressed in normal growth conditions. Disruption of this AtNUC-L1 gene leads to severe plant growth and development defects. AtNUC-L1 is localized in the nucleolus, mainly in the dense fibrillar component. Absence of this protein in Atnuc-L1 plants induces nucleolar disorganization, nucleolus organizer region decondensation, and affects the accumulation levels of pre-rRNA precursors. Remarkably, in Atnuc-L1 plants the AtNUC-L2 gene is activated, suggesting that AtNUC-L2 might rescue, at least partially, the loss of AtNUC-L1. This work is the first description of a higher eukaryotic organism with a disrupted nucleolin-like gene and defines a new role for nucleolin in nucleolus structure and rDNA chromatin organization.
The online version of this article contains supplemental material at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Julio Sáez-Vásquez (saez{at}univ-perp.fr)
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