![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on October 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 7, 2002
Revised on May 10, 2002
Accepted on July 8, 2002
1 Membrane Biology Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore
2 The Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Qld 4072, Australia
3 The Institute for Molecular Bioscience, University of Queensland, St. Lucia, Brisbane, Qld 4072, Australia; and Diabetes and Metabolism Research Program, Garvan Institute of Medical Research, St. Vincents Hospital, 384 Victoria St, Darlinghurst, NSW 2010, Sydney, Australia
* Corresponding author. E-mail address: mcbhwj{at}imcb.nus.edu.sg.
The subcellular localization, interacting partners, and function of GS15, a Golgi SNARE, remain to be established. In our present study, it is revealed that unlike proteins (Bet1 and the KDEL receptor) cycling between the Golgi and the intermediate compartment (IC, inclusive of the ER exit sites), GS15 is not redistributed into the IC upon incubation at 15 °C or when cells are treated with brefeldin A. Immuno-electron microscopy (immuno-EM) reveals that GS15 is mainly found in the medial-cisternae of the Golgi apparatus and adjacent tubulo-vesicular elements. Co-immunoprecipitation experiments suggest that GS15 exists in a distinct SNARE complex that contains SNAREs (syntaxin5, GS28 and Ykt6) that are implicated in both ER-to-Golgi and intra-Golgi transport but not with SNAREs involved exclusively in ER-to-Golgi traffic. Furthermore, components of COPI coat can be selectively co-immunoprecipitated with GS15 from Golgi extracts. Overexpression of mutant forms of GS15 affects the normal distribution of cis- and medial-Golgi proteins (GS28, syntaxin 5 and Golgi mannosidase II), whereas proteins of the trans-Golgi and TGN (Vti1-rp2/Vti1a and syntaxin 6) and Golgi matrix/scaffold (GM130 and p115) are less affected. When the level of GS15 is reduced by duplex 21-nt small interfering RNA (siRNA)-mediated knockdown approach, diverse markers of the Golgi apparatus are redistributed into small dotty and diffuse labeling, suggesting an essential role of GS15 in the Golgi apparatus.
This article has been cited by other articles:
|
|
 |
|
  Y. Guo, V. Punj, D. Sengupta, and A. D. Linstedt Coat-Tether Interaction in Golgi Organization Mol. Biol. Cell, July 1, 2008; 19(7): 2830 - 2843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tang, K. Mar, G. Warren, and Y. Wang Molecular Mechanism of Mitotic Golgi Disassembly and Reassembly Revealed by a Defined Reconstitution Assay J. Biol. Chem., March 7, 2008; 283(10): 6085 - 6094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Z. Lieu, M. C. Derby, R. D. Teasdale, C. Hart, P. Gunn, and P. A. Gleeson The Golgin GCC88 Is Required for Efficient Retrograde Transport of Cargo from the Early Endosomes to the Trans-Golgi Network Mol. Biol. Cell, December 1, 2007; 18(12): 4979 - 4991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Jollivet, G. Raposo, A. Dimitrov, R. Sougrat, B. Goud, and F. Perez Analysis of De Novo Golgi Complex Formation after Enzyme-based Inactivation Mol. Biol. Cell, November 1, 2007; 18(11): 4637 - 4647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Sehgal, S. Mukhopadhyay, F. Xu, K. Patel, and M. Shah Dysfunction of Golgi tethers, SNAREs, and SNAPs in monocrotaline-induced pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1526 - L1542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Amessou, A. Fradagrada, T. Falguieres, J. M. Lord, D. C. Smith, L. M. Roberts, C. Lamaze, and L. Johannes Syntaxin 16 and syntaxin 5 are required for efficient retrograde transport of several exogenous and endogenous cargo proteins J. Cell Sci., April 15, 2007; 120(8): 1457 - 1468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. T. Tran, Q. Zeng, and W. Hong VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes J. Cell Sci., March 15, 2007; 120(6): 1028 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sztul and V. Lupashin Role of tethering factors in secretory membrane traffic Am J Physiol Cell Physiol, January 1, 2006; 290(1): C11 - C26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Cosson, M. Ravazzola, O. Varlamov, T. H. Sollner, M. Di Liberto, A. Volchuk, J. E. Rothman, and L. Orci Dynamic transport of SNARE proteins in the Golgi apparatus PNAS, October 11, 2005; 102(41): 14647 - 14652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Graf, D. Riedel, H. D. Schmitt, and R. Jahn Identification of Functionally Interacting SNAREs by Using Complementary Substitutions in the Conserved `0' Layer Mol. Biol. Cell, May 1, 2005; 16(5): 2263 - 2274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Weng, Y. Li, D. Xu, Y. Shi, and H. Tang Specific Cleavage of Mcl-1 by Caspase-3 in Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis in Jurkat Leukemia T Cells J. Biol. Chem., March 18, 2005; 280(11): 10491 - 10500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Karabekian, L. K. Hanson, J. S. Slater, N. K. Krishna, L. L. Bolin, J. A. Kerry, and A. E. Campbell Complex Formation among Murine Cytomegalovirus US22 Proteins Encoded by Genes M139, M140, and M141 J. Virol., March 15, 2005; 79(6): 3525 - 3535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Zolov and V. V. Lupashin Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells J. Cell Biol., February 28, 2005; 168(5): 747 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Lee, J.-S. Yang, W. Hong, R. T. Premont, and V. W. Hsu ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation J. Cell Biol., January 17, 2005; 168(2): 281 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Tai, L. Lu, T. L. Wang, B. L. Tang, B. Goud, L. Johannes, and W. Hong Participation of the Syntaxin 5/Ykt6/GS28/GS15 SNARE Complex in Transport from the Early/Recycling Endosome to the Trans-Golgi Network Mol. Biol. Cell, September 1, 2004; 15(9): 4011 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, D. Ungar, F. M. Hughson, and M. Krieger The COG and COPI Complexes Interact to Control the Abundance of GEARs, a Subset of Golgi Integral Membrane Proteins Mol. Biol. Cell, May 1, 2004; 15(5): 2423 - 2435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack Mol. Biol. Cell, April 1, 2004; 15(4): 1506 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zeng, T. T. H. Tran, H.-X. Tan, and W. Hong The Cytoplasmic Domain of Vamp4 and Vamp5 Is Responsible for Their Correct Subcellular Targeting: THE N-TERMINAL EXTENSION OF VAMP4 CONTAINS A DOMINANT AUTONOMOUS TARGETING SIGNAL FOR THE TRANS-GOLGI NETWORK J. Biol. Chem., June 13, 2003; 278(25): 23046 - 23054. [Abstract] [Full Text] [PDF]
|
|
