![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on July 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 29, 2002
Revised on March 15, 2002
Accepted on April 19, 2002
1 Adolf-Butenandt-Institut/Zellbiologie, Ludwig-Maximilians, Universität München, Schillerstrasse 42, D-80336 München, Germany
* Corresponding author. E-mail address: Ralph.Graef{at}lrz.uni-muenchen.de.
EB1 proteins are ubiquitous microtubule-associated proteins involved in microtubule search-and-capture, regulation of microtubule dynamics, cell polarity and chromosome stability. We have cloned a complete cDNA of Dictyostelium EB1 (DdEB1), the largest known EB1 homologue (57 kDa). Immunofluorescence analysis and expression of a GFP-DdEB1 fusion protein revealed that DdEB1 localizes along microtubules, at microtubule tips, centrosomes and protruding pseudopods. During mitosis, it was found at the spindle, spindle poles and kinetochores. DdEB1 is the first EB1-homologue which is also a genuine centrosomal component, because it was localized at isolated centrosomes that are free of microtubules. Furthermore, centrosomal DdEB1 distribution was unaffected by nocodazole treatment. DdEB1 colocalized with DdCP224, the XMAP215 homologue, at microtubule tips, the centrosome and kinetochores. Furthermore, both proteins were part of the same cytosolic protein complex suggesting that they may act together in their functions. DdEB1 deletion mutants expressed as GFP or maltose-binding fusion proteins indicated that microtubule binding requires homo-oligomerization which is mediated by a coiled-coil domain. A DdEB1 null mutant was viable but retarded in prometaphase progression due to a defect in spindle formation. As spindle elongation was normal, DdEB1 seems to be required for the initiation of the outgrowth of spindle microtubules.
This article has been cited by other articles:
|
|
 |
|
  K. Brandner, A. Sambade, E. Boutant, P. Didier, Y. Mely, C. Ritzenthaler, and M. Heinlein Tobacco Mosaic Virus Movement Protein Interacts with Green Fluorescent Protein-Tagged Microtubule End-Binding Protein 1 Plant Physiology, June 1, 2008; 147(2): 611 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Bisgrove, Y.-R. J. Lee, B. Liu, N. T. Peters, and D. L. Kropf The Microtubule Plus-End Binding Protein EB1 Functions in Root Responses to Touch and Gravity Signals in Arabidopsis PLANT CELL, February 1, 2008; 20(2): 396 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Itoh and S. Yumura A novel mitosis-specific dynamic actin structure in Dictyostelium cells J. Cell Sci., December 15, 2007; 120(24): 4302 - 4309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rohlfs, R. Arasada, P. Batsios, J. Janzen, and M. Schleicher The Ste20-like kinase SvkA of Dictyostelium discoideum is essential for late stages of cytokinesis J. Cell Sci., December 15, 2007; 120(24): 4345 - 4354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kaller, U. Euteneuer, and W. Nellen Differential Effects of Heterochromatin Protein 1 Isoforms on Mitotic Chromosome Distribution and Growth in Dictyostelium discoideum Eukaryot. Cell, March 1, 2006; 5(3): 530 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chan, G. Calder, S. Fox, and C. Lloyd Localization of the Microtubule End Binding Protein EB1 Reveals Alternative Pathways of Spindle Development in Arabidopsis Suspension Cells PLANT CELL, June 1, 2005; 17(6): 1737 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Browning and D. D. Hackney The EB1 Homolog Mal3 Stimulates the ATPase of the Kinesin Tea2 by Recruiting It to the Microtubule J. Biol. Chem., April 1, 2005; 280(13): 12299 - 12304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Slep, S. L. Rogers, S. L. Elliott, H. Ohkura, P. A. Kolodziej, and R. D. Vale Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end J. Cell Biol., February 14, 2005; 168(4): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Elliott, C. F. Cullen, N. Wrobel, M. J. Kernan, and H. Ohkura EB1 Is Essential during Drosophila Development and Plays a Crucial Role in the Integrity of Chordotonal Mechanosensory Organs Mol. Biol. Cell, February 1, 2005; 16(2): 891 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Bisgrove, W. E. Hable, and D. L. Kropf +TIPs and Microtubule Regulation. The Beginning of the Plus End in Plants Plant Physiology, December 1, 2004; 136(4): 3855 - 3863. [Full Text] [PDF]
|
|
|
|
|
|
A. Kerres, C. Vietmeier-Decker, J. Ortiz, I. Karig, C. Beuter, J. Hegemann, J. Lechner, and U. Fleig The Fission Yeast Kinetochore Component Spc7 Associates with the EB1 Family Member Mal3 and Is Required for Kinetochore-Spindle Association Mol. Biol. Cell, December 1, 2004; 15(12): 5255 - 5267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brumby, J. Secombe, J. Horsfield, M. Coombe, N. Amin, D. Coates, R. Saint, and H. Richardson A Genetic Screen for Dominant Modifiers of a cyclin E Hypomorphic Mutation Identifies Novel Regulators of S-Phase Entry in Drosophila Genetics, September 1, 2004; 168(1): 227 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Bu and L.-K. Su Characterization of Functional Domains of Human EB1 Family Proteins J. Biol. Chem., December 12, 2003; 278(50): 49721 - 49731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Graf, U. Euteneuer, T.-H. Ho, and M. Rehberg Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number Mol. Biol. Cell, October 1, 2003; 14(10): 4067 - 4074. [Abstract] [Full Text] [PDF]
|
|
