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MBC in Press, published online ahead of print August 6, 2002
Mol. Biol. Cell 10.1091/mbc.E02-03-0127

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Submitted on March 6, 2002
Revised on June 27, 2002
Accepted on July 18, 2002

An endogenous RNA transcript antisense to CNG{alpha}1 cation channel mRNA

Chin-Hung Cheng1, David Tai-Wai Yew2, Hiu-Yee Kwan1, Qing Zhou1, Yu Huang1, Yong Liu3, Wing-Yee Chan3, and Xiaoqiang Yao1*

1 Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
2 Department of Anatomy, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
3 Department of Anatomical and Cellular Pathology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

* Corresponding author. E-mail address: yao2068{at}cuhk.edu.hk.

CNG channels are cyclic nucleotide-gated Ca2+-permeable channels that are suggested to be involved in the activity-dependent alterations of synaptic strength which is thought to underlie information storage in central nervous system. In this study, we isolated an endogenous RNA transcript antisense to CNG{alpha}1 mRNA. This transcript was capable of down-regulating the expression of sense CNG{alpha}1 in Xenopus oocyte expression system. RT-PCR, Northern blot and in situ hybridization analyses showed that the transcript was co-expressed with CNG{alpha}1 mRNA in many regions of human brain, notably in those regions that were involved in the long-term potentiation and long-term depression such as hippocampal CA1 and CA3, dentate gyrus and cerebellar Purkinje layer. Comparison of expression patterns between adult and fetal cerebral cortex revealed that there were concurrent developmental changes in the expression levels of antiCNG1 and CNG{alpha}1. Treatment of human glioma cell T98 with thyroid hormone T3 caused a significant increase in antiCNG1 expression and a parallel decrease in sense CNG{alpha}1 expression. These data suggest that the suppression of CNG{alpha}1 expression by antiCNG1 may play an important role in neuronal functions, especially in synaptic plasticity and cortical development. Endogenous antisense RNA-mediated regulation may represent a new mechanism through which the activity of ion channels can be regulated in human central nervous system.






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