Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 16, 2002
Mol. Biol. Cell 10.1091/mbc.E02-03-0172

A more recent version of this article appeared on September 1, 2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E02-03-0172v1
13/9/3162    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hua, Z.
Right arrow Articles by Graham, T. R.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hua, Z.
Right arrow Articles by Graham, T. R.

Submitted on March 28, 2002
Revised on May 23, 2002
Accepted on June 5, 2002

An Essential Subfamily of Drs2p-Related P-Type ATPases are Required for Protein Trafficking between the Golgi Complex and the Endosomal/Vacuolar System

Zhaolin Hua1, Parvin Fatheddin1, and Todd R. Graham1*

1 Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235-1634

* Corresponding author. E-mail address: tr.graham{at}vanderbilt.edu.

The Saccharomyces cerevisiae genome contains five genes encoding P-type ATPases that are potential aminophospholipid translocases (APTs); DRS2, NEO1, and three uncharacterized ORFs, which we have named DNF1, DNF2 and DNF3 for DRS2/NEO1 Family. NEO1 is the only essential gene in APT family and appears to be functionally distinct from the DRS2/DNF genes. The drs2{Delta} dnf1{Delta} dnf2{Delta} dnf3{Delta} quadruple mutant is inviable although any one member of this group can maintain viability, indicating that there is a substantial functional overlap between the encoded proteins. We have previously implicated Drs2p in clathrin function at the trans-Golgi network (TGN). In this study, we constructed strains carrying all possible viable combinations of null alleles from this group and analyzed them for defects in protein transport. The drs2{Delta} dnf1{Delta} mutant grows slowly, massively accumulates intracellular membranes, and exhibits a substantial defect in the transport of alkaline phosphatase (ALP) to the vacuole. Transport of carboxypeptidase Y (CPY) to the vacuole is also perturbed, but to a lesser extent. In addition, the dnf1{Delta} dnf2{Delta} dnf3{Delta} mutant exhibits a defect in recycling of GFP-Snc1p in the early endocytic - late secretory pathways. Drs2p and Dnf3p colocalize with the TGN marker Kex2p, while Dnf1p and Dnf2p appear to localize to the plasma membrane and late exocytic or early endocytic membranes. We propose that eukaryotes express multiple APT subfamily members to facilitate protein transport in multiple pathways.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
K. Liu, K. Surendhran, S. F. Nothwehr, and T. R. Graham
P4-ATPase Requirement for AP-1/Clathrin Function in Protein Transport from the trans-Golgi Network and Early Endosomes
Mol. Biol. Cell, August 1, 2008; 19(8): 3526 - 3535.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Ikeda, A. Kihara, A. Denpoh, and Y. Igarashi
The Rim101 Pathway Is Involved in Rsb1 Expression Induced by Altered Lipid Asymmetry
Mol. Biol. Cell, May 1, 2008; 19(5): 1922 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Nakano, T. Yamamoto, T. Kishimoto, T. Noji, and K. Tanaka
Protein Kinases Fpk1p and Fpk2p are Novel Regulators of Phospholipid Asymmetry
Mol. Biol. Cell, April 1, 2008; 19(4): 1783 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L. R. Poulsen, R. L. Lopez-Marques, S. C. McDowell, J. Okkeri, D. Licht, A. Schulz, T. Pomorski, J. F. Harper, and M. G. Palmgren
The Arabidopsis P4-ATPase ALA3 Localizes to the Golgi and Requires a {beta}-Subunit to Function in Lipid Translocation and Secretory Vesicle Formation
PLANT CELL, March 1, 2008; 20(3): 658 - 676.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul
Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking
J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. K. Schroeder, S. Kremer, M. J. Kramer, E. Currie, E. Kwan, J. L. Watts, A. L. Lawrenson, and G. J. Hermann
Function of the Caenorhabditis elegans ABC Transporter PGP-2 in the Biogenesis of a Lysosome-related Fat Storage Organelle
Mol. Biol. Cell, March 1, 2007; 18(3): 995 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Liu, Z. Hua, J. A. Nepute, and T. R. Graham
Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway
Mol. Biol. Cell, February 1, 2007; 18(2): 487 - 500.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. L. Daleke
Phospholipid Flippases
J. Biol. Chem., January 12, 2007; 282(2): 821 - 825.
[Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Furuta, K. Fujimura-Kamada, K. Saito, T. Yamamoto, and K. Tanaka
Endocytic Recycling in Yeast Is Regulated by Putative Phospholipid Translocases and the Ypt31p/32p-Rcy1p Pathway
Mol. Biol. Cell, January 1, 2007; 18(1): 295 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Alder-Baerens, Q. Lisman, L. Luong, T. Pomorski, and J. C.M. Holthuis
Loss of P4 ATPases Drs2p and Dnf3p Disrupts Aminophospholipid Transport and Asymmetry in Yeast Post-Golgi Secretory Vesicles
Mol. Biol. Cell, April 1, 2006; 17(4): 1632 - 1642.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Kishimoto, T. Yamamoto, and K. Tanaka
Defects in Structural Integrity of Ergosterol and the Cdc50p-Drs2p Putative Phospholipid Translocase Cause Accumulation of Endocytic Membranes, onto Which Actin Patches Are Assembled in Yeast
Mol. Biol. Cell, December 1, 2005; 16(12): 5592 - 5609.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
S W C van Mil, R H J Houwen, and L W J Klomp
Genetics of familial intrahepatic cholestasis syndromes
J. Med. Genet., June 1, 2005; 42(6): 449 - 463.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Kihara and Y. Igarashi
Cross Talk between Sphingolipids and Glycerophospholipids in the Establishment of Plasma Membrane Asymmetry
Mol. Biol. Cell, November 1, 2004; 15(11): 4949 - 4959.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Wicky, H. Schwarz, and B. Singer-Kruger
Molecular Interactions of Yeast Neo1p, an Essential Member of the Drs2 Family of Aminophospholipid Translocases, and Its Role in Membrane Trafficking within the Endomembrane System
Mol. Cell. Biol., September 1, 2004; 24(17): 7402 - 7418.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Bugnicourt, M. Froissard, K. Sereti, H. D. Ulrich, R. Haguenauer-Tsapis, and J.-M. Galan
Antagonistic Roles of ESCRT and Vps Class C/HOPS Complexes in the Recycling of Yeast Membrane Proteins
Mol. Biol. Cell, September 1, 2004; 15(9): 4203 - 4214.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Natarajan, J. Wang, Z. Hua, and T. R. Graham
Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function
PNAS, July 20, 2004; 101(29): 10614 - 10619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Saito, K. Fujimura-Kamada, N. Furuta, U. Kato, M. Umeda, and K. Tanaka
Cdc50p, a Protein Required for Polarized Growth, Associates with the Drs2p P-Type ATPase Implicated in Phospholipid Translocation in Saccharomyces cerevisiae
Mol. Biol. Cell, July 1, 2004; 15(7): 3418 - 3432.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Pomorski, J. C. M. Holthuis, A. Herrmann, and G. van Meer
Tracking down lipid flippases and their biological functions
J. Cell Sci., February 22, 2004; 117(6): 805 - 813.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Chantalat, S.-K. Park, Z. Hua, K. Liu, R. Gobin, A. Peyroche, A. Rambourg, T. R. Graham, and C. L. Jackson
The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae
J. Cell Sci., February 15, 2004; 117(5): 711 - 722.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Perez-Victoria, F. Gamarro, M. Ouellette, and S. Castanys
Functional Cloning of the Miltefosine Transporter: A NOVEL P-TYPE PHOSPHOLIPID TRANSLOCASE FROM LEISHMANIA INVOLVED IN DRUG RESISTANCE
J. Biol. Chem., December 12, 2003; 278(50): 49965 - 49971.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Z. Hua and T. R. Graham
Requirement for Neo1p in Retrograde Transport from the Golgi Complex to the Endoplasmic Reticulum
Mol. Biol. Cell, December 1, 2003; 14(12): 4971 - 4983.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. K. Hanson, L. Malone, J. L. Birchmore, and J. W. Nichols
Lem3p Is Essential for the Uptake and Potency of Alkylphosphocholine Drugs, Edelfosine and Miltefosine
J. Biol. Chem., September 19, 2003; 278(38): 36041 - 36050.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Pomorski, R. Lombardi, H. Riezman, P. F. Devaux, G. van Meer, and J. C. M. Holthuis
Drs2p-related P-type ATPases Dnf1p and Dnf2p Are Required for Phospholipid Translocation across the Yeast Plasma Membrane and Serve a Role in Endocytosis
Mol. Biol. Cell, March 1, 2003; 14(3): 1240 - 1254.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2002 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.