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A more recent version of this article appeared on November 1, 2002
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Submitted on April 16, 2002
Revised on July 2, 2002
Accepted on August 8, 2002
-tubulin is an essential component of the centriole
1 Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110; and Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309
2 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110
3 Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309
4 Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110
* Corresponding author. E-mail address: dutcher{at}genetics.wustl.edu.
Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2 kb fragment that contains 
-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate or lysine. Polyclonal antibodies to -tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, -tubulin is encoded by the BLD2 allele and -tubulin plays a role in basal body/centriole morphogenesis.
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