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A more recent version of this article appeared on November 1, 2002
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Submitted on May 10, 2002
Revised on June 24, 2002
Accepted on July 23, 2002
1 Central Clinical Chemistry Laboratory, Geneva University Hospital, Rue Micheli-du-Crest 24, 1211 Genève 14, Switzerland
2 INSERM U369, Faculté de Médecine Lyon-R.T.H. Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France
* Corresponding author. E-mail address: diaz{at}laennec.univ-lyon1.fr.
The notion of a "plurifunctional" nucleolus is now well established. However, molecular mechanisms underlying the biological processes occurring within this nuclear domain remain only partially understood. As a first step in elucidating these mechanisms we have carried out a proteomic analysis to draw up a list of proteins present within nucleoli of HeLa cells. This analysis allowed the identification of 213 different nucleolar proteins. This catalogue complements that of the 271 proteins obtained recently by others (Andersen et al., 2002, Curr Biol, 12, 1-11) giving a total of about 350 different nucleolar proteins. Functional classification of these proteins allowed outlining several biological processes taking place within nucleoli. Bioinformatic analyses permitted the assignment of hypothetical functions for 43 proteins for which no functional information is available. Notably, a role in ribosome biogenesis was proposed for 31 proteins. More generally, this functional classification reinforces the plurifunctional nature of nucleoli and provides convincing evidence that nucleoli may play a central role in the control of gene expression. Finally, this analysis supports the recent demonstration of a coupling of transcription and translation in higher eukaryotes.
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