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A more recent version of this article appeared on March 1, 2003
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Submitted on June 3, 2002
Revised on October 2, 2002
Accepted on November 6, 2002
1 LSA-335/3200, University of California, Berkeley, Berkeley, CA 94720-3200
2 Bayer Pharmaceutical Division, Berkeley, CA 94701-1986
3 University of the Pacific School of Pharmacy, Stockton, CA 95211
4 Ingenuity Inc., Mountain View, CA
5 LSA-335/3200, University of California, Berkeley, CA 94720-3200
* Corresponding author. E-mail address: acdose{at}socrates.berkeley.edu.
The striped bass has two retina-eexpressed class III myosin genes, each comprised of a kinase, motor and tail domain. We report the cloning, sequence analysis and expression patterns of the long (Myo3A) and short (Myo3B) class III myosins, as well as cellular localization and biochemical characterization of the long isoform, Myo3A. Myo3A, (209 kD) is expressed in the retina, brain, testis and sacculus, and Myo3B, (155 kD), is expressed in the retina, intestine and testis. The tails of these two isoforms contain two highly conserved domains, 3THDI and 3THDII. While Myo3B has three IQ motifs, Myo3A has nine IQ motifs, four in its neck and 5 in its tail domain. Myo3A localizes to actin filament bundles of photoreceptors and is concentrated in the calycal processes. An anti-MYO3A antibody decorates the actin cytoskeleton of rod inner/outer segments, and this labeling is reduced by the presence of ATP. The ATP-sensitive actin association is a feature characteristic of myosin motors. The numerous IQ motifs may play a stuctural or signaling role in the Myo3A and its localization to calycal processes (abbreviated as CPs) indicates that this myosin mediates a local function at this site in vertebrate photoreceptors.
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