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MBC in Press, published online ahead of print September 24, 2002
Mol. Biol. Cell 10.1091/mbc.E02-06-0332

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Submitted on June 10, 2002
Revised on July 30, 2002
Accepted on August 8, 2002

Meiotic cohesion requires accumulation of ORD on chromosomes prior to condensation

Eric M. Balicky1, Matthew W. Endres2, Cary Lai3, and Sharon E. Bickel1*

1 Department of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, New Hampshire 03755-3576
2 Department of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, New Hampshire 03755-3576 (present address: Department of Molecular, Cellular, and Developmental Biology, University of South Carolina, 700 Sumter Street, Columbia, South Carolina 29205)
3 Department of Biology, MIT and Whitehead Institute, 9 Cambridge Center, Cambridge, Massachusetts 02142 (present address: Department of Molecular and Cellular Biology, University of California, Berkeley, California 94720)

* Corresponding author. E-mail address: s.bickel{at}dartmouth.edu.

Cohesion between sister chromatids is a prerequisite for accurate chromosome segregation during mitosis and meiosis. To allow chromosome condensation during prophase, the connections that hold sister chromatids together must be maintained but still permit extensive chromatin compaction. In Drosophila, null mutations in the ord gene lead to meiotic nondisjunction in males and females because cohesion is absent by the time that sister kinetochores make stable microtubule attachments. We provide evidence that ORD is concentrated within the extrachromosomal domains of the nuclei of Drosophila primary spermatocytes during early G2 but accumulates on the meiotic chromosomes by mid to late G2. Moreover, using FISH to monitor cohesion directly, we show that cohesion defects first become detectable in ordnull spermatocytes shortly after the time when wild-type ORD associates with the chromosomes. Following condensation, ORD remains bound at the centromeres of wild-type spermatocytes and persists there until centromeric cohesion is released during anaphase II. Our results suggest that association of ORD with meiotic chromosomes during mid to late G2 is required to maintain sister-chromatid cohesion during prophase condensation and that retention of ORD at the centromeres after condensation ensures the maintenance of centromeric cohesion until anaphase II.




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[Abstract] [Full Text] [PDF]


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