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A more recent version of this article appeared on April 1, 2003
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Submitted on June 14, 2002
Revised on November 18, 2002
Accepted on December 4, 2002
1 Haartman Institute, Department of Virology and Biomedicum Helsinki, Programme for Developmental and Reproductive Biology, Haartmaninkatu 8, P.O. Box 63, FIN-00014 University of Helsinki, Finland
2 Institute for Medical Technology, FIN-33014 University of Tampere, Finland, and Research Unit, Tampere University Hospital
3 Haartman Institute, Department of Virology and Biomedicum Helsinki, Programme for Developmental and Reproductive Biology, Haartmaninkatu 8, P.O. Box 63, FIN-00014 University of Helsinki, Finland; and Institute for Medical Technology, FIN-33014 University of Tampere, Finland; and Department of Clinical Microbiology, Tampere University Hospital, P.O. Box 607, FIN-33101 Tampere, Finland
* Corresponding author. E-mail address: ltolsi{at}uta.fi.
Jak tyrosine kinases have a unique domain structure containing a kinase domain (JH1) adjacent to a catalytically inactive pseudokinase domain (JH2). JH2 is crucial for inhibition of basal Jak activity, but the mechanism of this regulation has remained elusive. We show that JH2 negatively regulated Jak2 in bacterial cells, indicating that regulation is an intrinsic property of Jak2. JH2 suppressed basal Jak2 activity by lowering the Vmax of Jak2, while JH2 did not affect the Km of Jak2 for a peptide substrate. Three inhibitory regions (IR1-3) within JH2 were identified. IR3 (residues 758-807), at the C-terminus of JH2, directly inhibited JH1 suggesting an inhibitory interaction between IR3 and JH1. Molecular modeling of JH2 showed that IR3 could form a stable 
-helical fold, supporting that IR3 could independently inhibit JH1. IR2 (725-757) in the C-terminal lobe of JH2, and IR1 (619-670) extending from the N-terminal to the C-terminal lobe enhanced IR3-mediated inhibition of JH1. Disruption of IR3 either by mutations or a small deletion increased basal Jak2 activity, but abolished IFN- 
-inducible signaling. Altogether, the results provide evidence for autoinhibition of a Jak family kinase and identify JH2 regions important for autoregulation of Jak2.
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