![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on January 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on April 1, 2002
Revised on August 1, 2002
Accepted on October 3, 2002
1 Department of Developmental Biology, Stanford University School of Medicine, Stanford CA 94305-5329
2 Istituto Pasteur Fondazionie Cenci Bolognetti and Centro di Genetica Evoluzionistica del CNR, Dipartimento di Genetica e Biologia Molecolare, Universitá "La Sapienza," Rome, Italy
3 Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford CA 94305-5329
* Corresponding author. E-mail address: fuller{at}cmgm.stanford.edu.
The multisubunit conserved oligomeric Golgi (COG) complex has been previously shown to be involved in Golgi function in yeast and mammalian tissue culture cells. Despite this broad conservation, several subunits, including Cog5, were not essential for growth and showed only mild effects on secretion when mutated in yeast, raising questions about what functions these COG complex subunits play in the life of the cell. Here we show that function of the gene four way stop (fws), which encodes the Drosophila Cog5 homolog, is necessary for dramatic changes in cellular and subcellular morphology during spermatogenesis. Loss of function mutations in fws caused failure of cleavage furrow ingression in dividing spermatocytes, failure of cell elongation in differentiating spermatids, and disrupted the formation and/or stability of the Golgi-based spermatid acroblast. Consistent with the lack of a growth defect in yeast lacking Cog5, animals lacking fws function were viable, although males were sterile. Fws protein localized to Golgi structures throughout spermatogenesis. We propose that Fws may directly or indirectly facilitate efficient vesicle traffic through the Golgi to support rapid and extensive increases in cell surface area during spermatocyte cytokinesis and polarized elongation of differentiating spermatids. Our study suggests that Drosophila spermatogenesis can be an effective sensitized genetic system to uncover in vivo functions for proteins involved in Golgi architecture and/or vesicle transport.
This article has been cited by other articles:
|
|
 |
|
  J. Cao, R. Albertson, B. Riggs, C. M. Field, and W. Sullivan Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization J. Cell Biol., July 28, 2008; 182(2): 301 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Wei, J. Rollins, L. Fabian, M. Hayes, G. Polevoy, C. Bazinet, and J. A. Brill Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis J. Cell Sci., April 1, 2008; 121(7): 1076 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dyer, E. Rebollo, P. Dominguez, N. Elkhatib, P. Chavrier, L. Daviet, C. Gonzalez, and M. Gonzalez-Gaitan Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes Development, December 15, 2007; 134(24): 4437 - 4447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Giansanti, G. Belloni, and M. Gatti Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males Mol. Biol. Cell, December 1, 2007; 18(12): 5034 - 5047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Riggs, B. Fasulo, A. Royou, S. Mische, J. Cao, T. S. Hays, and W. Sullivan The Concentration of Nuf, a Rab11 Effector, at the Microtubule-organizing Center Is Cell Cycle regulated, Dynein-dependent, and Coincides with Furrow Formation Mol. Biol. Cell, September 1, 2007; 18(9): 3313 - 3322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kranz, B. G. Ng, L. Sun, V. Sharma, E. A. Eklund, Y. Miura, D. Ungar, V. Lupashin, R. D. Winkel, J. F. Cipollo, et al. COG8 deficiency causes new congenital disorder of glycosylation type IIh Hum. Mol. Genet., April 1, 2007; 16(7): 731 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Coutelis and A. Ephrussi Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis Development, April 1, 2007; 134(7): 1419 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lee and L. Cooley Jagunal is required for reorganizing the endoplasmic reticulum during Drosophila oogenesis J. Cell Biol., March 26, 2007; 176(7): 941 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steet and S. Kornfeld COG-7-deficient Human Fibroblasts Exhibit Altered Recycling of Golgi Proteins Mol. Biol. Cell, May 1, 2006; 17(5): 2312 - 2321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Foulquier, E. Vasile, E. Schollen, N. Callewaert, T. Raemaekers, D. Quelhas, J. Jaeken, P. Mills, B. Winchester, M. Krieger, et al. Conserved oligomeric Golgi complex subunit 1 deficiency reveals a previously uncharacterized congenital disorder of glycosylation type II. PNAS, March 7, 2006; 103(10): 3764 - 3769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kubota, M. Sano, S. Goda, N. Suzuki, and K. Nishiwaki The conserved oligomeric Golgi complex acts in organ morphogenesis via glycosylation of an ADAM protease in C. elegans Development, January 15, 2006; 133(2): 263 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Emoto, H. Inadome, Y. Kanaho, S. Narumiya, and M. Umeda Local Change in Phospholipid Composition at the Cleavage Furrow Is Essential for Completion of Cytokinesis J. Biol. Chem., November 11, 2005; 280(45): 37901 - 37907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, E. Vasile, M. Penman, C. D. Novina, D. M. Dykxhoorn, D. Ungar, F. M. Hughson, and M. Krieger Genetic Analysis of the Subunit Organization and Function of the Conserved Oligomeric Golgi (COG) Complex: STUDIES OF COG5- AND COG7-DEFICIENT MAMMALIAN CELLS J. Biol. Chem., September 23, 2005; 280(38): 32736 - 32745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Fotso, Y. Koryakina, O. Pavliv, A. B. Tsiomenko, and V. V. Lupashin Cog1p Plays a Central Role in the Organization of the Yeast Conserved Oligomeric Golgi Complex J. Biol. Chem., July 29, 2005; 280(30): 27613 - 27623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, F. Peter, V. N. Subramaniam, and W. Hong Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus J. Cell Sci., March 15, 2005; 118(6): 1209 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Zolov and V. V. Lupashin Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells J. Cell Biol., February 28, 2005; 168(5): 747 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Oka and M. Krieger Multi-Component Protein Complexes and Golgi Membrane Trafficking J. Biochem., February 1, 2005; 137(2): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Herpers and C. Rabouille mRNA Localization and ER-based Protein Sorting Mechanisms Dictate the Use of Transitional Endoplasmic Reticulum-Golgi Units Involved in Gurken Transport in Drosophila Oocytes Mol. Biol. Cell, December 1, 2004; 15(12): 5306 - 5317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Saul, L. Fabian, A. Forer, and J. A. Brill Continuous phosphatidylinositol metabolism is required for cleavage of crane fly spermatocytes J. Cell Sci., August 1, 2004; 117(17): 3887 - 3896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh and W. Hong The Binary Interacting Network of the Conserved Oligomeric Golgi Tethering Complex J. Biol. Chem., June 4, 2004; 279(23): 24640 - 24648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. T. Wakimoto, D. L. Lindsley, and C. Herrera Toward a Comprehensive Genetic Analysis of Male Fertility in Drosophila melanogaster Genetics, May 1, 2004; 167(1): 207 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Oka, D. Ungar, F. M. Hughson, and M. Krieger The COG and COPI Complexes Interact to Control the Abundance of GEARs, a Subset of Golgi Integral Membrane Proteins Mol. Biol. Cell, May 1, 2004; 15(5): 2423 - 2435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Giansanti, R. M. Farkas, S. Bonaccorsi, D. L. Lindsley, B. T. Wakimoto, M. T. Fuller, and M. Gatti Genetic Dissection of Meiotic Cytokinesis in Drosophila Males Mol. Biol. Cell, May 1, 2004; 15(5): 2509 - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ghosh-Roy, M. Kulkarni, V. Kumar, S. Shirolikar, and K. Ray Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila Mol. Biol. Cell, May 1, 2004; 15(5): 2470 - 2483. [Abstract] [Full Text] [PDF]
|
|
