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A more recent version of this article appeared on January 1, 2003
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Submitted on June 30, 2002
Revised on October 4, 2002
Accepted on October 10, 2002
1 Department of Biochemistry and Molecular Biology
Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130
2 Department of Biochemistry and Molecular Biology
Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130 (present address: SUNY-Buffalo, Department of Biochemistry, 140 Farber Hall, Buffalo, NY 14214)
3 Department of Biochemistry and Molecular Biology
Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130 (present address: Division of Basic Science Fred Hutchinson Cancer Research Center, 1100 Fairfiew Ave, North, Seattle, WA 98105)
4 Department of Biochemistry and Molecular Biology
Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130 (present address: GlaxoSmithKline, 709 Swedeland Road, PO Box 1539, King of Prussia, PA 19406)
* Corresponding author. E-mail address: ktatch{at}lsuhsc.edu.
Bni4 is a scaffold protein in the yeast Saccharomyces cerevisiae that tethers chitin synthase III to the bud neck by interacting with septin neck filaments and with Chs4, a regulatory subunit of chitin synthase III. We show here that Bni4 is also a limiting determinant for the targeting of the type 1 serine/threonine phosphatase (Glc7) to the bud neck. Yeast cells containing a Bni4 variant that fails to associate with Glc7 fail to tether Chs4 to the neck, due in part to the failure of Bni4V831A/F833A to localize properly. Conversely, the Glc7-129 mutant protein fails to bind Bni4 properly and glc7-129 mutants exhibit reduced levels of Bni4 at the bud neck. Bni4 is phosphorylated in a cell cycle-dependent manner and Bni4V831A/F833A is both hyperphosphorylated and mislocalized in vivo. Yeast cells lacking the protein kinase Hsl1 exhibit increased levels of Bni4-GFP at the bud neck. GFP-Chs4 does not accumulate at the incipient bud site in either a bni4::TRP1 or a bni4V831A/F833A mutant but does mobilize to the neck at cytokinesis. Together, these results indicate that the formation of the Bni4-Glc7 complex is required for localization to the site of bud emergence and for subsequent targeting of chitin synthase.
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