![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on January 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 3, 2002
Revised on September 10, 2002
Accepted on October 10, 2002
throughout the mammalian cell cycle
1 Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH UK
* Corresponding author. E-mail address: l.trinklemulcahy{at}dundee.ac.uk.
Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. When transiently expressed as fluorescent protein (FP) fusions, the three PP1 isoforms, 
, 
/
and 
1, are active phosphatases with distinct localization patterns. We report here the establishment and characterization of HeLa cell lines stably expressing either FP-PP1 or FP alone. Time-lapse imaging reveals dynamic targeting of FP-PP1 to specific sites throughout the cell cycle, contrasting with the diffuse pattern observed for FP alone. FP-PP1 shows a nucleolar accumulation during interphase. Upon entry into mitosis, it localizes initially at kinetochores, where it exchanges rapidly with the diffuse cytoplasmic pool. A dramatic relocalization of PP1 to the chromosome-containing regions occurs at the transition from early to late anaphase, and by telophase FP-PP1 also accumulates at the cleavage furrow and midbody. The changing spatio-temporal distribution of PP1 revealed using the stable PP1 cell lines implicates it in multiple processes, including nucleolar function, the regulation of chromosome segregation and cytokinesis.
This article has been cited by other articles:
|
|
 |
|
  J. Whyte, J. R. Bader, S. B.F. Tauhata, M. Raycroft, J. Hornick, K. K. Pfister, W. S. Lane, G. K. Chan, E. H. Hinchcliffe, P. S. Vaughan, et al. Phosphorylation regulates targeting of cytoplasmic dynein to kinetochores during mitosis J. Cell Biol., November 25, 2008; 183(5): 819 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, C. Cronmiller, and D. L. Brautigan Maternal Phosphatase Inhibitor-2 Is Required for Proper Chromosome Segregation and Mitotic Synchrony During Drosophila Embryogenesis Genetics, August 1, 2008; 179(4): 1823 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Bharucha, J. R. Larson, L. Gao, L. K. Daves, and K. Tatchell Ypi1, a Positive Regulator of Nuclear Protein Phosphatase Type 1 Activity in Saccharomyces cerevisiae Mol. Biol. Cell, March 1, 2008; 19(3): 1032 - 1045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Stamm Regulation of Alternative Splicing by Reversible Protein Phosphorylation J. Biol. Chem., January 18, 2008; 283(3): 1223 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Gunawardena, B. L. Ruis, J. A. Meyer, M. Kapoor, and K. F. Conklin NOM1 Targets Protein Phosphatase I to the Nucleolus J. Biol. Chem., January 4, 2008; 283(1): 398 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Novoyatleva, B. Heinrich, Y. Tang, N. Benderska, M. E.R. Butchbach, C. L. Lorson, M. A. Lorson, C. Ben-Dov, P. Fehlbaum, L. Bracco, et al. Protein phosphatase 1 binds to the RNA recognition motif of several splicing factors and regulates alternative pre-mRNA processing Hum. Mol. Genet., January 1, 2008; 17(1): 52 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Matic, M. van Hagen, J. Schimmel, B. Macek, S. C. Ogg, M. H. Tatham, R. T. Hay, A. I. Lamond, M. Mann, and A. C. O. Vertegaal In Vivo Identification of Human Small Ubiquitin-like Modifier Polymerization Sites by High Accuracy Mass Spectrometry and an in Vitro to in Vivo Strategy Mol. Cell. Proteomics, January 1, 2008; 7(1): 132 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Alvarez-Tabares, A. Grallert, J.-M. Ortiz, and I. M. Hagan Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth J. Cell Sci., October 15, 2007; 120(20): 3589 - 3601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Evangelisti, P. L. Tazzari, M. Riccio, R. Fiume, Y. Hozumi, F. Fala, K. Goto, L. Manzoli, L. Cocco, and A. M. Martelli Nuclear diacylglycerol kinase-{zeta} is a negative regulator of cell cycle progression in C2C12 mouse myoblasts FASEB J, October 1, 2007; 21(12): 3297 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kirchner, S. Gross, D. Bennett, and L. Alphey Essential, Overlapping and Redundant Roles of the Drosophila Protein Phosphatase 1{alpha} and 1{beta} Genes Genetics, May 1, 2007; 176(1): 273 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Pinsky, C. V. Kotwaliwale, S. Y. Tatsutani, C. A. Breed, and S. Biggins Glc7/Protein phosphatase 1 regulatory subunits can oppose the ipl1/aurora protein kinase by redistributing glc7. Mol. Cell. Biol., April 1, 2006; 26(7): 2648 - 2660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Trinkle-Mulcahy, J. Andersen, Y. W. Lam, G. Moorhead, M. Mann, and A. I. Lamond Repo-Man recruits PP1{gamma} to chromatin and is essential for cell viability J. Cell Biol., February 27, 2006; 172(5): 679 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Los, F. P. Vinke, J. de Widt, M. K. Topham, W. J. van Blitterswijk, and N. Divecha The Retinoblastoma Family Proteins Bind to and Activate Diacylglycerol Kinase{zeta} J. Biol. Chem., January 13, 2006; 281(2): 858 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. CHUSAINOW, P. M. AJUH, L. TRINKLE-MULCAHY, J. E. SLEEMAN, J. ELLENBERG, and A. I. LAMOND FRET analyses of the U2AF complex localize the U2AF35/U2AF65 interaction in vivo and reveal a novel self-interaction of U2AF35 RNA, August 1, 2005; 11(8): 1201 - 1214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Szeszel, C. L. Crisman, L. Crow, S. McMullen, J. M. Major, L. Natarajan, A. Saquib, J. R. Feramisco, and L. M. Wasserman Quantifying Estrogen and Progesterone Receptor Expression in Breast Cancer by Digital Imaging J. Histochem. Cytochem., June 1, 2005; 53(6): 753 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. W. Lam, L. Trinkle-Mulcahy, and A. I. Lamond The nucleolus J. Cell Sci., April 1, 2005; 118(7): 1335 - 1337. [Full Text] [PDF]
|
|
|
|
|
|
G. M. Wilson, A. B. Fielding, G. C. Simon, X. Yu, P. D. Andrews, R. S. Hames, A. M. Frey, A. A. Peden, G. W. Gould, and R. Prekeris The FIP3-Rab11 Protein Complex Regulates Recycling Endosome Targeting to the Cleavage Furrow during Late Cytokinesis Mol. Biol. Cell, February 1, 2005; 16(2): 849 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lesage, M. Beullens, M. Nuytten, A. Van Eynde, S. Keppens, B. Himpens, and M. Bollen Interactor-mediated Nuclear Translocation and Retention of Protein Phosphatase-1 J. Biol. Chem., December 31, 2004; 279(53): 55978 - 55984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Maiato, J. DeLuca, E. D. Salmon, and W. C. Earnshaw The dynamic kinetochore-microtubule interface J. Cell Sci., November 1, 2004; 117(23): 5461 - 5477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hrabchak and S. Varmuza Identification of the Spermatogenic Zip Protein Spz1 as a Putative Protein Phosphatase-1 (PP1) Regulatory Protein That Specifically Binds the PP1c{gamma}2 Splice Variant in Mouse Testis J. Biol. Chem., August 27, 2004; 279(35): 37079 - 37086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. O. Vertegaal, S. C. Ogg, E. Jaffray, M. S. Rodriguez, R. T. Hay, J. S. Andersen, M. Mann, and A. I. Lamond A Proteomic Study of SUMO-2 Target Proteins J. Biol. Chem., August 6, 2004; 279(32): 33791 - 33798. [Abstract] [Full Text] [PDF]
|
|
