![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on May 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 18, 2002
Revised on December 4, 2002
Accepted on January 16, 2003
1 UMR 144 CNRS/Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
* Corresponding author. E-mail address: marpin{at}curie.fr.
Ezrin, a membrane cytoskeleton linker, is involved in cellular functions including epithelial cell morphogenesis and adhesion. A mutant form of ezrin, ezrin T567D, maintains the protein in an open conformation, which when expressed in MDCK cells causes extensive formation of lamellipodia and altered cell-cell contacts at low cell density. Furthermore, these cells do not form tubules when grown in a collagen type I matrix. While measuring the activity of Rho family GTPases, we found that Rac1, but not RhoA or Cdc 42, is activated in ezrin T567D expressing cells, compared to cells expressing wild type ezrin. Together with Rac1 activation, we observed an accumulation of E-cadherin in intracellular compartments and a concomitant decrease in the level of E-cadherin present at the plasma membrane. This effect could be reversed with a dominant negative form of Rac1, N17Rac1. We show that after a calcium switch, the delivery of E-cadherin from an internalized pool to the plasma membrane is greatly delayed in ezrin T567D producing cells. In confluent cells, ezrin T567D production decreases the rate of E-cadherin internalization. Our results identify a new role for ezrin in cell adhesion through the activation of the GTPase Rac1 and the trafficking of E-cadherin to the plasma membrane.
This article has been cited by other articles:
|
|
 |
|
  C. Zahn, A. Jaschke, J. Weiske, A. Hommel, D. Hesse, R. Augustin, L. Lu, W. Hong, S. Florian, A. Scheepers, et al. ADP-ribosylation Factor-like GTPase ARFRP1 Is Required for Trans-Golgi to Plasma Membrane Trafficking of E-cadherin J. Biol. Chem., October 3, 2008; 283(40): 27179 - 27188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D'Angelo, S. Aresta, A. Blangy, L. Del Maestro, D. Louvard, and M. Arpin Interaction of Ezrin with the Novel Guanine Nucleotide Exchange Factor PLEKHG6 Promotes RhoG-dependent Apical Cytoskeleton Rearrangements in Epithelial Cells Mol. Biol. Cell, December 1, 2007; 18(12): 4780 - 4793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, B. S. Emerald, S. Mukhina, K. M. Mohankumar, A. Kraemer, A. S. Yap, P. D. Gluckman, K.-O. Lee, and P. E. Lobie HOXA1 Is Required for E-cadherin-dependent Anchorage-independent Survival of Human Mammary Carcinoma Cells J. Biol. Chem., March 10, 2006; 281(10): 6471 - 6481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Casey, P. J. Neville, X. Liu, S. J. Plummer, M. S. Cicek, L. M. Krumroy, A. P. Curran, M. R. McGreevy, W. J. Catalona, E. A. Klein, et al. Podocalyxin variants and risk of prostate cancer and tumor aggressiveness Hum. Mol. Genet., March 1, 2006; 15(5): 735 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Yang and P. W. Hinds Phosphorylation of ezrin by cyclin-dependent kinase 5 induces the release of rho GDP dissociation inhibitor to inhibit rac1 activity in senescent cells. Cancer Res., March 1, 2006; 66(5): 2708 - 2715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koss, G. R. Pfeiffer II, Y. Wang, S. T. Thomas, M. Yerukhimovich, W. A. Gaarde, C. M. Doerschuk, and Q. Wang Ezrin/Radixin/Moesin Proteins Are Phosphorylated by TNF-{alpha} and Modulate Permeability Increases in Human Pulmonary Microvascular Endothelial Cells J. Immunol., January 15, 2006; 176(2): 1218 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liu and T. A. Voyno-Yasenetskaya Radixin Stimulates Rac1 and Ca2+/Calmodulin-dependent Kinase, CaMKII: CROSS-TALK WITH G{alpha}13 SIGNALING J. Biol. Chem., November 25, 2005; 280(47): 39042 - 39049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Srivastava, B. E. Elliott, D. Louvard, and M. Arpin Src-dependent Ezrin Phosphorylation in Adhesion-mediated Signaling Mol. Biol. Cell, March 1, 2005; 16(3): 1481 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. van Buul and P. L. Hordijk Signaling in Leukocyte Transendothelial Migration Arterioscler. Thromb. Vasc. Biol., May 1, 2004; 24(5): 824 - 833. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. N. Wrobel, J. Debnath, E. Lin, S. Beausoleil, M. F. Roussel, and J. S. Brugge Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture J. Cell Biol., April 26, 2004; 165(2): 263 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Fievet, A. Gautreau, C. Roy, L. Del Maestro, P. Mangeat, D. Louvard, and M. Arpin Phosphoinositide binding and phosphorylation act sequentially in the activation mechanism of ezrin J. Cell Biol., March 1, 2004; 164(5): 653 - 659. [Abstract] [Full Text] [PDF]
|
|
