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MBC in Press, published online ahead of print December 25, 2002
Mol. Biol. Cell 10.1091/mbc.E02-08-0500

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Submitted on August 14, 2002
Revised on November 6, 2002
Accepted on December 4, 2002

Role of microtubules in the fusion of post-Golgi vesicles to the plasma membrane

Jan Schmoranzer1 and Sanford M. Simon2*

1 Laboratory of Cellular Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10021; and Department of Biology, Chemistry, Pharmacology, Free University Berlin, Berlin, Germany
2 Laboratory of Cellular Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10021

* Corresponding author. E-mail address: simon{at}mail.rockefeller.edu.

Biosynthetic cargo is transported away from the Golgi in vesicles via microtubules. In the cell periphery the vesicles are believed to engage actin and then dock to fusion sites at the plasma membrane. Using dual-color total internal reflection fluorescence microscopy (TIR-FM) we observed that microtubules extended within 100 nm of the plasma membrane and post-Golgi vesicles remained on microtubules up to the plasma membrane, even as fusion to the plasma membrane initiated. Disruption of microtubules eliminated the tubular shapes of the vesicles and altered the fusion events: vesicles required multiple fusions to deliver all of their membrane cargo to the plasma membrane. In contrast, the effects of disrupting actin on fusion behavior were subtle. We conclude that microtubules, rather than actin filaments, are the cytoskeletal elements on which post-Golgi vesicles are transported until they fuse to the plasma membrane.




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