Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print July 11, 2003
Mol. Biol. Cell 10.1091/mbc.E02-12-0781

A more recent version of this article appeared on September 1, 2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E02-12-0781v1
14/9/3888    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lecuona, E.
Right arrow Articles by Sznajder, J. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lecuona, E.
Right arrow Articles by Sznajder, J. I.

Submitted on December 2, 2002
Revised on May 1, 2003
Accepted on May 2, 2003

The GTP-binding Protein RhoA Mediates Na,K-ATPase Exocytosis in Alveolar Epithelial Cells

Emilia Lecuona1*, Karen Ridge1, Liuska Pesce1, Daniel Batlle2, and Jacob I. Sznajder1

1 Division of Pulmonary and Critical Care Medicine
2 Division of Nephrology, Department of Medicine, Northwestern University. Chicago, Illinois, 60611

* Corresponding author. E-mail address: e-lecuona{at}northwestern.edu.

The purpose of this study was to define the role of the Rho family of small GTPases in the {beta}-adrenergic regulation of the Na,K-ATPase in alveolar epithelial cells (AEC). The {beta}-adrenergic receptor agonist isoproterenol (ISO) increased the Na,K-ATPase protein abundance at the plasma membrane and activated RhoA in a time-dependent manner. AEC pretreated with mevastatin, a specific inhibitor of prenylation, or transfected with the dominant negative RhoAN19, prevented ISO-mediated Na,K-ATPase exocytosis to the plasma membrane. The ISO-mediated activation of RhoA in AEC occurred via {beta}2-adrenergic receptor activation of RhoA and involved Gs-PKA as demonstrated by incubation with the PKA specific inhibitors H89 and PKI (peptide specific inhibitor), and Gi, as incubation with pertussis toxin or cells transfected with a minigene vector for Gi inhibited the ISO-mediated RhoA activation. However, cells transfected with minigene vectors for G12 and G13 did not prevent RhoA activation by ISO. Finally, the ISO-mediated Na,K-ATPase exocytosis was regulated by the Rho-associated kinase (ROCK), as preincubation with the specific inhibitor Y-27632 or transfection with dominant negative ROCK, prevented the increase in Na,K-ATPase at the plasma membrane. Accordingly, ISO regulates Na,K-ATPase exocytosis in alveolar epithelial cells via the activation of {beta}2-adrenergic receptor, Gs, PKA, Gi, RhoA and ROCK.




This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. M. Mutlu and P. Factor
Alveolar Epithelial 2-Adrenergic Receptors
Am. J. Respir. Cell Mol. Biol., February 1, 2008; 38(2): 127 - 134.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W. Schoner and G. Scheiner-Bobis
Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C509 - C536.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Shen, M.-J. Lin, A. Yaradanakul, V. Lariccia, J. A. Hill, and D. W. Hilgemann
Dual control of cardiac Na+ Ca2+ exchange by PIP2: analysis of the surface membrane fraction by extracellular cysteine PEGylation
J. Physiol., August 1, 2007; 582(3): 1011 - 1026.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. A. Dada, E. Novoa, E. Lecuona, H. Sun, and J. I. Sznajder
Role of the small GTPase RhoA in the hypoxia-induced decrease of plasma membrane Na,K-ATPase in A549 cells
J. Cell Sci., July 1, 2007; 120(13): 2214 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Lecuona, L. A. Dada, H. Sun, M. L. Butti, G. Zhou, T.-L. Chew, and J. I. Sznajder
Na,K-ATPase {alpha}1-subunit dephosphorylation by protein phosphatase 2A is necessary for its recruitment to the plasma membrane
FASEB J, December 1, 2006; 20(14): 2618 - 2620.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
Z. S. Azzam, Y. Adir, L. Welch, J. Chen, J. Winaver, P. Factor, N. Krivoy, A. Hoffman, J. I. Sznajder, and Z. Abassi
Alveolar fluid reabsorption is increased in rats with compensated heart failure
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L1094 - L1100.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. Suzuki, Z.-G. Jin, D. F. Meoli, T. Matoba, and B. C. Berk
Cyclophilin A Is Secreted by a Vesicular Pathway in Vascular Smooth Muscle Cells
Circ. Res., March 31, 2006; 98(6): 811 - 817.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
G. M. Mutlu and J. I. Sznajder
Mechanisms of pulmonary edema clearance
Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L685 - L695.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2003 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.