![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on July 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 10, 2003
Revised on March 11, 2003
Accepted on March 11, 2003
-catenin regulation during the cell cycle. Implications in G2/M and apoptosis
1 Instituto de Investigaciones Biomédicas 'Alberto Sols', Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, 28029 Madrid, Spain
2 Servicios Científico-Técnicos, Universidad de Barcelona, Universidad de Barcelona, 08028 Barcelona, Spain
3 Departamento de Biología Celular, Universidad de Barcelona, Universidad de Barcelona, 08028 Barcelona, Spain
* Corresponding author. E-mail address: acano{at}iib.uam.es.
-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. -catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems -catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of -catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous -catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase synchronized epithelial cells. -catenin levels increase in S phase reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of -catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not -catenin, is detected in M phase. Interestingly, overexpression of a stable form of -catenin, or inhibition of endogenous -catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for -catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes.
This article has been cited by other articles:
|
|
 |
|
  Y. Wang, Y. Azuma, D. Moore, N. Osheroff, and K. L. Neufeld Interaction between Tumor Suppressor Adenomatous Polyposis Coli and Topoisomerase II{alpha}: Implication for the G2/M Transition Mol. Biol. Cell, October 1, 2008; 19(10): 4076 - 4085. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Boyer, L. Hermo, M. Paquet, B. Robaire, and D. Boerboom Seminiferous Tubule Degeneration and Infertility in Mice with Sustained Activation of WNT/CTNNB1 Signaling in Sertoli Cells Biol Reprod, September 1, 2008; 79(3): 475 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Hayes, D. Thomas, A. Emmons, T. J. Giordano, and C. G. Kleer Genetic Changes of Wnt Pathway Genes Are Common Events in Metaplastic Carcinomas of the Breast Clin. Cancer Res., July 1, 2008; 14(13): 4038 - 4044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Aoki and M. M. Taketo Adenomatous polyposis coli (APC): a multi-functional tumor suppressor gene J. Cell Sci., October 1, 2007; 120(19): 3327 - 3335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Ghaleb, B. B. McConnell, M. O. Nandan, J. P. Katz, K. H. Kaestner, and V. W. Yang Haploinsufficiency of Kruppel-Like Factor 4 Promotes Adenomatous Polyposis Coli Dependent Intestinal Tumorigenesis Cancer Res., August 1, 2007; 67(15): 7147 - 7154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-T. Teh, D. Blaydon, L. R. Ghali, S. Edmunds, E. Pantazi, M. R. Barnes, I. M. Leigh, D. P. Kelsell, and M. P. Philpott Role for WNT16B in human epidermal keratinocyte proliferation and differentiation J. Cell Sci., January 15, 2007; 120(2): 330 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. L. Pon and A. S. T. Wong Gonadotropin-Induced Apoptosis in Human Ovarian Surface Epithelial Cells Is Associated with Cyclooxygenase-2 Up-Regulation via the {beta}-Catenin/T-Cell Factor Signaling Pathway Mol. Endocrinol., December 1, 2006; 20(12): 3336 - 3350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bachar-Dahan, J. Goltzmann, A. Yaniv, and A. Gazit Engrailed-1 Negatively Regulates beta-Catenin Transcriptional Activity by Destabilizing beta-Catenin via a Glycogen Synthase Kinase-3beta-independent Pathway Mol. Biol. Cell, June 1, 2006; 17(6): 2572 - 2580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nair, A. Teng, V. Bilanchone, A. Agrawal, B. Li, and X. Dai Ovol1 regulates the growth arrest of embryonic epidermal progenitor cells and represses c-myc transcription J. Cell Biol., April 24, 2006; 173(2): 253 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Millan, D. Rico, H. Peinado, N. Zarich, K. Stamatakis, D. Perez-Sala, J. M. Rojas, A. Cano, and L. Bosca Potentiation of tumor formation by topical administration of 15-deoxy-{Delta}12,14-prostaglandin J2 in a model of skin carcinogenesis Carcinogenesis, February 1, 2006; 27(2): 328 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Ellison, O. E. Onilude, J. C. Lindsey, M. E. Lusher, C. L. Weston, R. E. Taylor, A. D. Pearson, and S. C. Clifford {beta}-Catenin Status Predicts a Favorable Outcome in Childhood Medulloblastoma: The United Kingdom Children's Cancer Study Group Brain Tumour Committee J. Clin. Oncol., November 1, 2005; 23(31): 7951 - 7957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Serban, Z. Kouchi, L. Baki, A. Georgakopoulos, C. M. Litterst, J. Shioi, and N. K. Robakis Cadherins Mediate Both the Association between PS1 and {beta}-Catenin and the Effects of PS1 on {beta}-Catenin Stability J. Biol. Chem., October 28, 2005; 280(43): 36007 - 36012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Li, T.-o Ishikawa, H. Miyoshi, M. Oshima, and M. M. Taketo A Targeted Mutation of Nkd1 Impairs Mouse Spermatogenesis J. Biol. Chem., January 28, 2005; 280(4): 2831 - 2839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Choi, S. Y. Park, F. Costantini, E.-h. Jho, and C.-K. Joo Adenomatous Polyposis Coli Is Down-regulated by the Ubiquitin-Proteasome Pathway in a Process Facilitated by Axin J. Biol. Chem., November 19, 2004; 279(47): 49188 - 49198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Kouzmenko, K.-i. Takeyama, S. Ito, T. Furutani, S. Sawatsubashi, A. Maki, E. Suzuki, Y. Kawasaki, T. Akiyama, T. Tabata, et al. Wnt/{beta}-Catenin and Estrogen Signaling Converge in Vivo J. Biol. Chem., September 24, 2004; 279(39): 40255 - 40258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Park, S. I. Kim, M. S. Lee, C.-y. Youn, D. J. Kim, E.-h. Jho, and W. K. Song Modulation of {beta}-Catenin Phosphorylation/Degradation by Cyclin-dependent Kinase 2 J. Biol. Chem., May 7, 2004; 279(19): 19592 - 19599. [Abstract] [Full Text] [PDF]
|
|
