Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print September 5, 2003
Mol. Biol. Cell 10.1091/mbc.E03-03-0169

A more recent version of this article appeared on November 1, 2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E03-03-0169v1
14/11/4365    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dalby-Payne, J. R.
Right arrow Articles by Gunning, P. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dalby-Payne, J. R.
Right arrow Articles by Gunning, P. P.

Submitted on March 24, 2003
Revised on July 20, 2003
Accepted on July 23, 2003

Polarisation of specific tropomyosin isoforms in gastrointestinal epithelial cells and their impact on CFTR at the apical surface

Jacqueline Rae Dalby-Payne1, Edward Vincent O'Loughlin1, and Professor Peter Gunning1*

1 Gastroenterology Research, The Children's Hospital at Westmead, Discipline of Paediatrics and Child Health, The University of Sydney, Sydney, Australia.; Gastroenterology Research, The Children's Hospital at Westmead, Westmead, Australia.; Oncology Research Unit, The Children's Hospital at Westmead, Discipline of Paediatrics and Child Health, The University of Sydney, Sydney, Australia

* Corresponding author. E-mail address: peterG3{at}chw.edu.au.

Microfilaments have been reported to be polarised in a number of cell types based both on function and isoform composition. There is evidence that microfilaments are involved in the movement of vesicles and the polarised delivery of proteins to specialized membrane domains. We have investigated the composition of actin microfilaments in gastrointestinal epithelial cells and their role in the delivery of the cystic fibrosis transmembrane conductance regulator (CFTR) into the apical membrane using cultured T84 cells as a model. We identified a specific population of microfilaments containing the tropomyosin (Tm) isoforms Tm5a and/or Tm5b, which are polarised in T84 cell monolayers. Polarization of this microfilament population occurs very rapidly in response to cell-cell and cell-substratum contact and is not inhibited by jasplakinolide suggesting this involves the movement of intact filaments. Colocalisation of Tm5a and/or Tm5b and CFTR was observed in long-term cultures. A reduction in Tm5a and Tm5b expression, induced using antisense oligonucleotides, resulted in an increase in both CFTR surface expression and chloride efflux in response to cAMP stimulation. We conclude that Tm isoforms Tm5a and/or Tm5b mark an apical population of microfilaments that can regulate the insertion and/or retention of CFTR into the plasma membrane.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
E.-M. S. Grimm-Gunter, C. Revenu, S. Ramos, I. Hurbain, N. Smyth, E. Ferrary, D. Louvard, S. Robine, and F. Rivero
Plastin 1 Binds to Keratin and Is Required for Terminal Web Assembly in the Intestinal Epithelium
Mol. Biol. Cell, May 15, 2009; 20(10): 2549 - 2562.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Nazeer, M. G. Janech, J. J.-C. Lin, K. J. Ryan, J. M. Arthur, and M. N. Budisavljevic
Changes in protein profiles during course of experimental glomerulonephritis
Am J Physiol Renal Physiol, January 1, 2009; 296(1): F186 - F193.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Vlahovich, A. J. Kee, C. Van der Poel, E. Kettle, D. Hernandez-Deviez, C. Lucas, G. S. Lynch, R. G. Parton, P. W. Gunning, and E. C. Hardeman
Cytoskeletal Tropomyosin Tm5NM1 Is Required for Normal Excitation-Contraction Coupling in Skeletal Muscle
Mol. Biol. Cell, January 1, 2009; 20(1): 400 - 409.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. Gunning, G. O'neill, and E. Hardeman
Tropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space
Physiol Rev, January 1, 2008; 88(1): 1 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Nazli, A. Wang, O. Steen, D. Prescott, J. Lu, M. H. Perdue, J. D. Soderholm, P. M. Sherman, and D. M. McKay
Enterocyte Cytoskeleton Changes Are Crucial for Enhanced Translocation of Nonpathogenic Escherichia coli across Metabolically Stressed Gut Epithelia
Infect. Immun., January 1, 2006; 74(1): 192 - 201.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
B. Vrhovski, K. McKay, G. Schevzov, P. W. Gunning, and R. P. Weinberger
Smooth Muscle-specific {alpha} Tropomyosin Is a Marker of Fully Differentiated Smooth Muscle in Lung
J. Histochem. Cytochem., July 1, 2005; 53(7): 875 - 883.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
G. Schevzov, B. Vrhovski, N. S. Bryce, S. Elmir, M. R. Qiu, G. M. O'Neill, N. Yang, N. M. Verrills, M. Kavallaris, and P. W. Gunning
Tissue-specific Tropomyosin Isoform Composition
J. Histochem. Cytochem., May 1, 2005; 53(5): 557 - 570.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. J. Kee, G. Schevzov, V. Nair-Shalliker, C. S. Robinson, B. Vrhovski, M. Ghoddusi, M. R. Qiu, J. J.-C. Lin, R. Weinberger, P. W. Gunning, et al.
Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy
J. Cell Biol., August 30, 2004; 166(5): 685 - 696.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2003 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.