![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on October 1, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on March 31, 2003
Revised on May 28, 2003
Accepted on May 28, 2003
1 Department of Immunoregulation, Research Institute for Microbial
Diseases, Osaka University, The Second Department of
Internal Medicine, Kumamoto University School of Medicine, Japan
* Corresponding author. E-mail address: tkinoshi{at}biken.osaka-u.ac.jp.
Many cell surface proteins are anchored to a membrane via a glycosylphosphatidylinositol (GPI), which is attached to the C termini in the endoplasmic reticulum. The inositol ring of phosphatidylinositol is acylated during biosynthesis of GPI. In mammalian cells, the acyl chain is added to glucosaminyl phosphatidylinositol at the third step in the GPI biosynthetic pathway, and then is usually removed soon after the attachment of GPIs to proteins. The mechanisms and roles of the inositol acylation and deacylation have not been well clarified. Here, we report derivation of human and Chinese hamster mutant cells defective in inositol acylation and the gene responsible, PIG-W. The surface expressions of GPI-anchored proteins on these mutant cells were greatly diminished, indicating the critical role of inositol acylation. PIG-W encodes a 504-amino-acid protein expressed in the endoplasmic reticulum. PIG-W is most likely inositol acyltransferase itself because the tagged PIG-W affinity-purified from transfected human cells had inositol acyltransferase activity and because both mutant cells were complemented with PIG-W homologues of S. cerevisiae and Schizosaccharomyces pombe. The inositol acylation is not essential for the subsequent mannosylation, indicating that glucosaminyl phosphatidylinositol can flip from the cytoplasmic side to the luminal side of the endoplasmic reticulum.
This article has been cited by other articles:
|
|
 |
|
  K. Kajiwara, R. Watanabe, H. Pichler, K. Ihara, S. Murakami, H. Riezman, and K. Funato Yeast ARV1 Is Required for Efficient Delivery of an Early GPI Intermediate to the First Mannosyltransferase during GPI Assembly and Controls Lipid Flow from the Endoplasmic Reticulum Mol. Biol. Cell, May 1, 2008; 19(5): 2069 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ueda, R. Yamaguchi, M. Ikawa, M. Okabe, E. Morii, Y. Maeda, and T. Kinoshita PGAP1 Knock-out Mice Show Otocephaly and Male Infertility J. Biol. Chem., October 19, 2007; 282(42): 30373 - 30380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Houjou, J. Hayakawa, R. Watanabe, Y. Tashima, Y. Maeda, T. Kinoshita, and R. Taguchi Changes in molecular species profiles of glycosylphosphatidylinositol anchor precursors in early stages of biosynthesis J. Lipid Res., July 1, 2007; 48(7): 1599 - 1606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Orlean and A. K. Menon Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids J. Lipid Res., May 1, 2007; 48(5): 993 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Okamoto, T. Yoko-o, M. Umemura, K.-i. Nakayama, and Y. Jigami Glycosylphosphatidylinositol-anchored Proteins Are Required for the Transport of Detergent-resistant Microdomain-associated Membrane Proteins Tat2p and Fur4p J. Biol. Chem., February 17, 2006; 281(7): 4013 - 4023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ashida, Y. Hong, Y. Murakami, N. Shishioh, N. Sugimoto, Y. U. Kim, Y. Maeda, and T. Kinoshita Mammalian PIG-X and Yeast Pbn1p Are the Essential Components of Glycosylphosphatidylinositol-Mannosyltransferase I Mol. Biol. Cell, March 1, 2005; 16(3): 1439 - 1448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pottekat and A. K. Menon Subcellular Localization and Targeting of N-Acetylglucosaminyl Phosphatidylinositol De-N-acetylase, the Second Enzyme in the Glycosylphosphatidylinositol Biosynthetic Pathway J. Biol. Chem., April 16, 2004; 279(16): 15743 - 15751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tanaka, Y. Maeda, Y. Tashima, and T. Kinoshita Inositol Deacylation of Glycosylphosphatidylinositol-anchored Proteins Is Mediated by Mammalian PGAP1 and Yeast Bst1p J. Biol. Chem., April 2, 2004; 279(14): 14256 - 14263. [Abstract] [Full Text] [PDF]
|
|
