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A more recent version of this article appeared on August 1, 2003
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Submitted on April 8, 2003
Accepted on April 11, 2003
1 Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
2 MRC Centre for Developmental Neurobiology, King's College London, New Hunts House, Guy's Campus, London SE1 1UL, UK
* Corresponding author. E-mail address: mcbsb{at}imcb.nus.edu.sg.
Mammalian LGN/AGS3 proteins and their Drosophila Pins orthologue are cytoplasmic regulators of G-protein signaling. In Drosophila, Pins localizes to the lateral cortex of polarized epithelial cells and to the apical cortex of neuroblasts where it plays important roles in their asymmetric division. Using overexpression studies in different cell line systems, we demonstrate here that, like Drosophila Pins, LGN can exhibit enriched localization at the cell cortex depending on the cell cycle and the culture system used. We find that in WISH, PC12, and NRK but not COS cells, LGN is largely directed to the cell cortex during mitosis. Overexpression of truncated Protein domains further identified the G
-binding C-terminal portion of LGN as a sufficient domain for cortical localization in cell culture. In mitotic COS cells which normally do not exhibit cortical LGN localization, LGN is redirected to the cell cortex upon overexpression of G subunits of heterotrimeric G-proteins. The results also show that the cortical localization of LGN is dependent on microfilaments and that interfering with LGN function in cultured cell lines causes early disruption to cell cycle progression.
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