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MBC in Press, published online ahead of print July 11, 2003
Mol. Biol. Cell 10.1091/mbc.E03-04-0260

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Submitted on April 27, 2003
Revised on June 9, 2003
Accepted on June 9, 2003

Nuclear pore protein gp210 is essential for viability in HeLa cells and C. elegans

Merav Cohen1, Naomi Feinstein1, Katherine L. Wilson2, and Yosef Gruenbaum1*

1 Department of Genetics, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904 Israel
2 Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore MD 21205 USA

* Corresponding author. E-mail address: gru{at}vms.huji.ac.il.

Gp210 is an evolutionarily conserved membrane protein of the nuclear pore complex (NPC). We studied the phenotypes produced by RNAi-induced down-regulation of gp210 in both human (HeLa) cells and C. elegans embryos. HeLa cell viability requires Gp210 activity. The dying cells accumulated clustered NPCs and aberrant membrane structures at the nuclear envelope, suggesting that gp210 is required directly or indirectly for nuclear pore formation and dilation, as well as the anchoring or structural integrity of mature NPCs. Essential roles for gp210 were confirmed in C. elegans, where RNAi-induced reduction of gp210 caused embryonic lethality. The nuclear envelopes of embryos with reduced gp210 also had aberrant nuclear membrane structures and clustered NPCs, confirming that gp210 plays critical roles at the nuclear membrane through mechanisms that are conserved from nematodes to humans.




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