![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on January 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on May 27, 2003
Revised on August 25, 2003
Accepted on August 26, 2003
1 European Molecular Biology Laboratory, Postfach 102209, D-69117 Heidelberg, Germany; European Molecular Biology Laboratory,
Meyerhoffstrasse 1, Postfach 102209, 69012 Heidelberg, Germany, These authors contributed equally to this study
2 European Molecular Biology Laboratory, Postfach 102209, D-69117 Heidelberg, Germany, These authors contributed equally to this study
3 European Molecular Biology Laboratory, Postfach 102209, D-69117 Heidelberg, Germany
4 Central Electron Microscopy Facility, Universität Ulm, Albert-Einstein-Allee 11, D-89069 Ulm, Germany
5 Institute of Cell Biology and Biosystems Technology, University of Rostock. D-18051 Rostock, Germany
* Corresponding author. E-mail address: griffiths{at}embl-heidelberg.de.
* Corresponding author. E-mail address: griffiths{at}embl-heidelberg.de.
Actin is implicated in membrane fusion but the precise mechanisms remain unclear. We showed earlier that membrane organelles catalyze the de novo assembly of F-actin that then facilitates the fusion between latex bead phagosomes (LBP) and a mixture of early and late endocytic organelles. Here, we correlated the polymerization and organization of F-actin with phagosome and endocytic organelle fusion processes in vitro using biochemistry, light- and electron-microscopy. When membrane organelles and cytosol were incubated at 37°C with ATP, cytosolic actin polymerized rapidly and became organized into bundles and networks adjacent to membrane organelles. By 30 mins incubation a gel-like state was formed with little further polymerization of actin thereafter. During this time also the bulk of in vitro fusion events occurred between phagosomes/endocytic organelles. The fusion between LBP and late endocytic organelles, or between late endocytic organelles themselves was facilitated by actin but we failed to detect any effect of perturbing F-actin polymerization on early endosome fusion. Consistent with this, late endosomes, like phagosomes, could nucleate F-actin, whereas early endosomes could not. We propose that actin assembled by phagosomes or late endocytic organelles can provide tracks for fusion-partner organelles to move vectorially toward them, via membrane-bound myosins, to faciliate fusion.
This article has been cited by other articles:
|
|
 |
|
  M. D. Lazarus, T. G. Schneider, and T. F. Taraschi A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum J. Cell Sci., June 1, 2008; 121(11): 1937 - 1949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Jutras, M. Houde, N. Currier, J. Boulais, S. Duclos, S. LaBoissiere, E. Bonneil, P. Kearney, P. Thibault, E. Paramithiotis, et al. Modulation of the Phagosome Proteome by Interferon-{gamma} Mol. Cell. Proteomics, April 1, 2008; 7(4): 697 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vincent, I. Maridonneau-Parini, C. Le Clainche, P. Gounon, and A. Labrousse Activation of p61Hck Triggers WASp- and Arp2/3-dependent Actin-comet Tail Biogenesis and Accelerates Lysosomes J. Biol. Chem., July 6, 2007; 282(27): 19565 - 19574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. M. Sehring, C. Reiner, J. Mansfeld, H. Plattner, and R. Kissmehl A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function J. Cell Sci., January 1, 2007; 120(1): 177 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Jacobs, L. V. DeSouza, H. Samaranayake, R. E. Pearlman, K. W. M. Siu, and L. A. Klobutcher The Tetrahymena thermophila Phagosome Proteome Eukaryot. Cell, December 1, 2006; 5(12): 1990 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Trivedi, S. C. Zhang, A. B. Castoreno, W. Stockinger, E. C. Shieh, J. M. Vyas, E.-M. Frickel, and A. Nohturfft Immunoglobulin G signaling activates lysosome/phagosome docking PNAS, November 28, 2006; 103(48): 18226 - 18231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Kalamidas, M. P. Kuehnel, P. Peyron, V. Rybin, S. Rauch, O. B. Kotoulas, M. Houslay, B. A. Hemmings, M. G. Gutierrez, E. Anes, et al. cAMP synthesis and degradation by phagosomes regulate actin assembly and fusion events: consequences for mycobacteria. J. Cell Sci., September 1, 2006; 119(Pt 17): 3686 - 3694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Stockinger, S. C. Zhang, V. Trivedi, L. A. Jarzylo, E. C. Shieh, W. S. Lane, A. B. Castoreno, and A. Nohturfft Differential Requirements for Actin Polymerization, Calmodulin, and Ca2+ Define Distinct Stages of Lysosome/Phagosome Targeting Mol. Biol. Cell, April 1, 2006; 17(4): 1697 - 1710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Holtta-Vuori, F. Alpy, K. Tanhuanpaa, E. Jokitalo, A.-L. Mutka, and E. Ikonen MLN64 Is Involved in Actin-mediated Dynamics of Late Endocytic Organelles Mol. Biol. Cell, August 1, 2005; 16(8): 3873 - 3886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Sevrioukov, N. Moghrabi, M. Kuhn, and H. Kramer A Mutation in dVps28 Reveals a Link between a Subunit of the Endosomal Sorting Complex Required for Transport-I Complex and the Actin Cytoskeleton in Drosophila Mol. Biol. Cell, May 1, 2005; 16(5): 2301 - 2312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Scott, W. Dobson, R. J. Botelho, N. Coady-Osberg, P. Chavrier, D. A. Knecht, C. Heath, P. Stahl, and S. Grinstein Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis J. Cell Biol., April 11, 2005; 169(1): 139 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Beaulieu, N. Da Silva, N. Pastor-Soler, C. R. Brown, P. J. S. Smith, D. Brown, and S. Breton Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+-ATPase Recycling J. Biol. Chem., March 4, 2005; 280(9): 8452 - 8463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kissmehl, I. M. Sehring, E. Wagner, and H. Plattner Immunolocalization of Actin in Paramecium Cells J. Histochem. Cytochem., December 1, 2004; 52(12): 1543 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tedrick, T. Trischuk, R. Lehner, and G. Eitzen Enhanced Membrane Fusion in Sterol-enriched Vacuoles Bypasses the Vrp1p Requirement Mol. Biol. Cell, October 1, 2004; 15(10): 4609 - 4621. [Abstract] [Full Text] [PDF]
|
|
