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A more recent version of this article appeared on January 1, 2004
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Submitted on June 11, 2003
Revised on September 3, 2003
Accepted on September 24, 2003
1 Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655
* Corresponding author. E-mail address: Jeanne.Lawrence{at}umassmed.edu.
Previous studies have shown that in a given cell-type certain
active genes associate with SC35 domains, nuclear regions rich in RNA
metabolic factors and excluded from heterochromatin. This organization
is not seen for all active genes, therefore it is important to
determine whether and when this locus-specific organization arises
during development and differentiation of specific cell-types. Here we
investigate whether gene organization relative to SC35 domains is
cell-type specific by following several muscle and nonmuscle genes in
human fibroblasts, committed but proliferative myoblasts, and
terminally differentiated muscle. While no change was seen for other
loci, two muscle genes (Human 
-cardiac myosin heavy
chain and myogenin) became localized to the periphery of
an SC35 domain in terminally differentiated muscle nuclei, but not in
proliferative myoblasts or in fibroblasts. There was no apparent change
in gene localization relative to either the chromosome territory or the
heterochromatic compartment, thus the gene repositioning appeared to
occur specifically with respect to SC35 domains. This gene relocation
adjacent to a prominent SC35 domain was recapitulated in mouse 3T3
cells induced into myogenesis by introduction of MyoD. RESULTS
demonstrate a cell-type specific reorganization of specific
developmentally regulated loci relative to large domains of RNA
metabolic factors, which may facilitate developmental regulation of
genome expression.
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