![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on April 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 27, 2003
Revised on December 15, 2003
Accepted on December 15, 2003
1 Unidad de Investigación, Hospital Universitario de Canarias. Ofra s/n, La Cuesta, 38320 Tenerife, Spain; These authors contributed equally to this work
2 Centro de Investigación del Cáncer, CSIC/University of Salamanca. Campus Unamuno, 37007 Salamanca, Spain; These authors contributed equally to this work
3 Department of Biology, York University. Downsview, Ontario, M3J 1P3 Canada
4 Biocenter Oulu and Department of Biochemistry, University of Oulu. P.O. BOX 3000, FIN-90014 Finland
5 Division of Molecular Genetics, Department of Biochemistry, University of Oxford. South Parks Road, Oxford, OX1 3QU, UK
6 Biocenter Oulu and Department of Biochemistry, University of Oulu. P.O. BOX 3000, FIN-90014 Finland; Department of Biology, University of Joensuu. P.O. BOX 111, FIN-80101 Joensuu, Finland
7 Centro de Investigación del Cáncer, CSIC/University of Salamanca. Campus Unamuno, 37007 Salamanca, Spain
8 Unidad de Investigación, Hospital Universitario de Canarias. Ofra s/n, La Cuesta, 38320 Tenerife, Spain
* Corresponding author. E-mail address: rfreire{at}huc.es.
* Corresponding author. E-mail address: rfreire{at}huc.es.
Mammalian TopBP1 is a BRCT domain-containing protein whose function in mitotic cells is linked to replication and DNA damage checkpoint. Here, we study its possible role during meiosis in mice. TopBP1 foci are abundant during early prophase I and localize mainly to histone 
-H2AX positive domains, where DNA double strand breaks (required to initiate recombination) occur. Strikingly, TopBP1 showed an almost identical pattern to that of ATR, a PI3K-like kinase involved in mitotic DNA damage checkpoint. In the synapsis-defective Fkbp6-/- mouse, TopBP1 heavily stains unsynapsed regions of chromosomes. We also tested whether Schizosaccharomyces pombe Cut5 (the TopBP1 homologue) is playing a role in the meiotic recombination checkpoint, like spRad3, the ATR homologue. Indeed, we found that a cut5 mutation suppresses the checkpoint-dependent meiotic delay of a meiotic recombination defective mutant, indicating a direct role of the Cut5 protein in the meiotic checkpoint. Our findings suggest that ATR and TopBP1 monitor meiotic recombination and are required for activation of the meiotic recombination checkpoint.
This article has been cited by other articles:
|
|
 |
|
  S. K. Mahadevaiah, D. Bourc'his, D. G. de Rooij, T. H. Bestor, J. M.A. Turner, and P. S. Burgoyne Extensive meiotic asynapsis in mice antagonises meiotic silencing of unsynapsed chromatin and consequently disrupts meiotic sex chromosome inactivation J. Cell Biol., July 28, 2008; 182(2): 263 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Venere, A. Snyder, O. Zgheib, and T. D. Halazonetis Phosphorylation of ATR-Interacting Protein on Ser239 Mediates an Interaction with Breast-Ovarian Cancer Susceptibility 1 and Checkpoint Function Cancer Res., July 1, 2007; 67(13): 6100 - 6105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Moens, E. Marcon, J. S. Shore, N. Kochakpour, and B. Spyropoulos Initiation and resolution of interhomolog connections: crossover and non-crossover sites along mouse synaptonemal complexes J. Cell Sci., March 15, 2007; 120(6): 1017 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Taricani and T. S.F. Wang Rad4TopBP1, a Scaffold Protein, Plays Separate Roles in DNA Damage and Replication Checkpoints and DNA Replication Mol. Biol. Cell, August 1, 2006; 17(8): 3456 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Barchi, S. Mahadevaiah, M. Di Giacomo, F. Baudat, D. G. de Rooij, P. S. Burgoyne, M. Jasin, and S. Keeney Surveillance of Different Recombination Defects in Mouse Spermatocytes Yields Distinct Responses despite Elimination at an Identical Developmental Stage Mol. Cell. Biol., August 15, 2005; 25(16): 7203 - 7215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I Roig, P Robles, R Garcia, I Martinez-Flores, L. Cabero, J Egozcue, B Liebe, H Scherthan, and M Garcia Chromosome 18 pairing behavior in human trisomic oocytes. Presence of an extra chromosome extends bouquet stage Reproduction, May 1, 2005; 129(5): 565 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Pankratz and S. L. Forsburg Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest Mol. Biol. Cell, April 1, 2005; 16(4): 1651 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Di Giacomo, M. Barchi, F. Baudat, W. Edelmann, S. Keeney, and M. Jasin Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants PNAS, January 18, 2005; 102(3): 737 - 742. [Abstract] [Full Text] [PDF]
|
|
