![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on April 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 30, 2003
Revised on November 7, 2003
Accepted on December 8, 2003
1 Dept. of Biological Sciences, 111 Research Dr. Lehigh University, Bethlehem, PA 18015
* Corresponding author. E-mail address: lc07{at}lehigh.edu.
The XMAP215/Dis1 MAP family is thought to regulate microtubule plus end assembly in part by antagonizing the catastrophe-promoting function of kin I kinesins, yet XMAP215/Dis1 proteins localize to centrosomes. We probed the mitotic function of TOGp (human homolog of XMAP215/Dis1) using siRNA. Cells lacking TOGp assembled multipolar spindles, confirming results of Gergely et al. (2003). Eg5 motor activity was necessary to maintain the multipolar morphology. Depletion of TOGp decreased microtubule length and density in the spindle by 
20%. Depletion of MCAK, a kin I kinesin, increased MT lengths and density by 20%, but did not disrupt spindle morphology. Mitotic cells lacking both TOGp and MCAK formed bipolar and monopolar spindles, indicating that TOGp and MCAK contribute to spindle bipolarity, without major effects on MT stability. TOGp localized to centrosomes in the absence of MTs and depletion of TOGp resulted in centrosome fragmentation. TOGp depletion also disrupted MT minus end focus at the spindle poles, detected by localizations of NuMA and the p150 component of dynactin. The major functions of TOGp during mitosis are to focus MT minus ends at spindle poles, maintain centrosome integrity, and contribute to spindle bipolarity.
This article has been cited by other articles:
|
|
 |
|
  A. R. Barr and F. Gergely MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics Mol. Cell. Biol., December 1, 2008; 28(23): 7199 - 7211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, S. C. Ems-McClung, and C. E. Walczak Aurora A Phosphorylates MCAK to Control Ran-dependent Spindle Bipolarity Mol. Biol. Cell, July 1, 2008; 19(7): 2752 - 2765. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yang, X. Liu, Y. Yin, M. N. Fukuda, and J. Zhou Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis FASEB J, June 1, 2008; 22(6): 1960 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sherwood, R. Manbodh, C. Sheppard, and A. D. Chalmers RASSF7 Is a Member of a New Family of RAS Association Domain-containing Proteins and Is Required for Completing Mitosis Mol. Biol. Cell, April 1, 2008; 19(4): 1772 - 1782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Park, J.-E. Park, T. S. Karpova, N.-K. Soung, L.-R. Yu, S. Song, K. H. Lee, X. Xia, E. Kang, I. Dabanoglu, et al. Requirement for the Budding Yeast Polo Kinase Cdc5 in Proper Microtubule Growth and Dynamics Eukaryot. Cell, March 1, 2008; 7(3): 444 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Graser, Y.-D. Stierhof, and E. A. Nigg Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion J. Cell Sci., December 15, 2007; 120(24): 4321 - 4331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wordeman, M. Wagenbach, and G. von Dassow MCAK facilitates chromosome movement by promoting kinetochore microtubule turnover J. Cell Biol., December 3, 2007; 179(5): 869 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tedeschi, M. Ciciarello, R. Mangiacasale, E. Roscioli, W. M. Rensen, and P. Lavia RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells J. Cell Sci., November 1, 2007; 120(21): 3748 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Schneider, F. Essmann, A. Kletke, P. Rio, H. Hanenberg, W. Wetzel, K. Schulze-Osthoff, B. Nurnberg, and R. P. Piekorz The Transforming Acidic Coiled Coil 3 Protein Is Essential for Spindle-dependent Chromosome Alignment and Mitotic Survival J. Biol. Chem., October 5, 2007; 282(40): 29273 - 29283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Barr and F. Gergely Aurora-A: the maker and breaker of spindle poles J. Cell Sci., September 1, 2007; 120(17): 2987 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. H. Thein, J. Kleylein-Sohn, E. A. Nigg, and U. Gruneberg Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity J. Cell Biol., July 24, 2007; 178(3): 345 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang, H. Li, H. Hu, X. Yan, M. S. Waterman, H. Huang, and X. J. Zhou Systematic discovery of functional modules and context-specific functional annotation of human genome Bioinformatics, July 1, 2007; 23(13): i222 - i229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Perez de Castro, G. de Carcer, and M. Malumbres A census of mitotic cancer genes: new insights into tumor cell biology and cancer therapy Carcinogenesis, May 1, 2007; 28(5): 899 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Holmfeldt, S. Stenmark, and M. Gullberg Interphase-specific Phosphorylation-mediated Regulation of Tubulin Dimer Partitioning in Human Cells Mol. Biol. Cell, May 1, 2007; 18(5): 1909 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bonfils, N. Bec, B. Lacroix, M.-C. Harricane, and C. Larroque Kinetic Analysis of Tubulin Assembly in the Presence of the Microtubule-associated Protein TOGp J. Biol. Chem., February 23, 2007; 282(8): 5570 - 5581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Ems-McClung, K. M. Hertzer, X. Zhang, M. W. Miller, and C. E. Walczak The Interplay of the N- and C-Terminal Domains of MCAK Control Microtubule Depolymerization Activity and Spindle Assembly Mol. Biol. Cell, January 1, 2007; 18(1): 282 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wong and G. Fang HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture J. Cell Biol., June 19, 2006; 173(6): 879 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Wolyniak, K. Blake-Hodek, K. Kosco, E. Hwang, L. You, and T. C. Huffaker The Regulation of Microtubule Dynamics in Saccharomyces cerevisiae by Three Interacting Plus-End Tracking Proteins Mol. Biol. Cell, June 1, 2006; 17(6): 2789 - 2798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Al-Bassam, M. van Breugel, S. C. Harrison, and A. Hyman Stu2p binds tubulin and undergoes an open-to-closed conformational change. J. Cell Biol., March 27, 2006; 172(7): 1009 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Chen, H. Li, and A. De Lozanne Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis Mol. Biol. Cell, February 1, 2006; 17(2): 779 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Green, R. Wollman, and K. B. Kaplan APC and EB1 Function Together in Mitosis to Regulate Spindle Dynamics and Chromosome Alignment Mol. Biol. Cell, October 1, 2005; 16(10): 4609 - 4622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M.J. Hut, H. H. Kampinga, and O. C.M. Sibon Hsp70 Protects Mitotic Cells against Heat-induced Centrosome Damage and Division Abnormalities Mol. Biol. Cell, August 1, 2005; 16(8): 3776 - 3785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. O'Brien, A. J. Albee, L. Liu, W. Tao, P. Dobrzyn, S. B. Lizarraga, and C. Wiese The Xenopus TACC Homologue, Maskin, Functions in Mitotic Spindle Assembly Mol. Biol. Cell, June 1, 2005; 16(6): 2836 - 2847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Ganem and D. A. Compton The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK J. Cell Biol., August 16, 2004; 166(4): 473 - 478. [Abstract] [Full Text] [PDF]
|
|
