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A more recent version of this article appeared on February 1, 2004
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Submitted on August 8, 2003
Revised on September 15, 2003
Accepted on October 6, 2003
1 Genome Damage and Stability Centre, University of Sussex, Brighton, BN1 9RQ, UK
2 The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK
3 MRC Radiation and Genome Stability Unit, Harwell, Didcot, OXON, OX11 ORD, UK
* Corresponding author. E-mail address: T.Humphrey{at}har.mrc.ac.uk.
A coordinated transcriptional response to DNA damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) using DNA microarrays. We identified 
200 genes whose transcript levels were significantly altered at least twofold in response to 500Gy 
IR in a temporally defined manner. The majority of induced genes were Core Environmental Stress Response (CESR) genes, while the remaining genes define a transcriptional response to DNA damage in fission yeast. Surprisingly few DNA repair and checkpoint genes were transcriptionally modulated in response to IR. We define a role for the Stress Activated MAP kinase, StyI/Spc1, and the DNA damage checkpoint kinase, Rad3, in regulating CESR and IR-specific response genes, both independently and in concert. These findings suggest a complex network of regulatory pathways coordinate gene expression responses to IR in eukaryotes.
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