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A more recent version of this article appeared on February 1, 2004
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Submitted on August 14, 2003
Revised on September 23, 2003
Accepted on October 3, 2003
1 Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700, These authors contributed equally to this work
2 Department of Microbiology, University of Virginia Health System, Charlottesville VA 22908-0734, These authors contributed equally to this work
3 Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700, Laboratory of Gene Expression and Regulation, National Institute for Basic Biology, Myodaijicho, Okazaki 444-8585 JAPAN
4 Department of Biological Chemistry, University of California-Irvine, Irvine, California 92697-1700
5 Department of Microbiology, University of Virginia Health System, Charlottesville VA 22908-0734
* Corresponding author. E-mail address: mnomura{at}uci.edu.
Yeast cells entering into stationary phase decrease rRNA synthesis rate by decreasing both the number of active genes and the transcription rate of individual active genes. Using chromatin immunoprecipitation assays, we found that the association of RNA polymerase I with the promoter and the coding region of rDNA is decreased in stationary phase, but association of transcription factor UAF with the promoter is unchanged. Similar changes were also observed when growing cells were treated with rapamycin, which is known to inhibit the Tor signaling system. Rapamycin treatment also caused a decrease in the amount of Rrn3p-polymerase I complex, similar to stationary phase. Since recruitment of Pol I to the rDNA promoter is Rrn3p-dependent as shown in this work, these data suggest that the decrease in the transcription rate of individual active genes in stationary phase is achieved by the Tor signaling system acting at the Rrn3p-dependent polymerase recruitment step. EM chromatin spreading analyses of cells treated with rapamycin and cells in postlog phase confirm this conclusion and demonstrate that the Tor system does not participate in alteration of the number of active genes observed for cells entering into stationary phase.
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