![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on May 1, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 15, 2003
Revised on January 12, 2004
Accepted on January 27, 2004
1 Sunnybrook & Women’s College Health Sciences Centre; Department of Laboratory Medicine and Pathobiology, University of Toronto
2 Sunnybrook & Women’s College Health Sciences Centre; Department of Anaesthesia, University of Toronto
3 Sunnybrook & Women’s College Health Sciences Centre; Department of Radiation Oncology, University of Toronto
* Corresponding author. E-mail address: burton.yang{at}sw.ca.
The chondroitin sulfate proteoglycan versican is one of the major extracellular components in the developing and adult brain. Here we show that isoforms of versican play different roles in neuronal differentiation and neurite outgrowth. Expression of versican V1 isoform in PC12 cells induced complete differentiation, while expression of V2 induced an aborted differentiation accompanied by apoptosis. V1 promoted neurite outgrowth of hippocampal neurons but V2 failed to do so. V1 transfection enhanced expression of EGF receptor and integrins, and facilitated sustained ERK/MAPK phosphorylation. Blockade of the EGF receptor, 
1 integrin, or Src significantly inhibited neuronal differentiation. Finally, we demonstrated that versican V1 isoform also promoted differentiation of neural stem cells into neurons. Our results have implications for understanding how versican regulates neuronal development, function, and repair.
This article has been cited by other articles:
|
|
 |
|
  B. Hu, L. L. Kong, R. T. Matthews, and M. S. Viapiano The Proteoglycan Brevican Binds to Fibronectin after Proteolytic Cleavage and Promotes Glioma Cell Motility J. Biol. Chem., September 5, 2008; 283(36): 24848 - 24859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Read, M. Rahmani, S. Boroomand, S. Allahverdian, B. M. McManus, and P. S. Rennie Androgen Receptor Regulation of the Versican Gene through an Androgen Response Element in the Proximal Promoter J. Biol. Chem., November 2, 2007; 282(44): 31954 - 31963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakanishi, S. Aono, K. Hirano, Y. Kuroda, M. Ida, Y. Tokita, F. Matsui, and A. Oohira Identification of Neurite Outgrowth-promoting Domains of Neuroglycan C, a Brain-specific Chondroitin Sulfate Proteoglycan, and Involvement of Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways in Neuritogenesis J. Biol. Chem., August 25, 2006; 281(34): 24970 - 24978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Zacharias and U. Rauch Competition and cooperation between tenascin-R, lecticans and contactin 1 regulate neurite growth and morphology. J. Cell Sci., August 15, 2006; 119(Pt 16): 3456 - 3466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Y. Xiang, H. Dong, Y. Wan, J. Li, A. Yee, B. B. Yang, and W.-Y. Lu Versican G3 Domain Regulates Neurite Growth and Synaptic Transmission of Hippocampal Neurons by Activation of Epidermal Growth Factor Receptor J. Biol. Chem., July 14, 2006; 281(28): 19358 - 19368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Deepa, D. Carulli, C. Galtrey, K. Rhodes, J. Fukuda, T. Mikami, K. Sugahara, and J. W. Fawcett Composition of Perineuronal Net Extracellular Matrix in Rat Brain: A DIFFERENT DISACCHARIDE COMPOSITION FOR THE NET-ASSOCIATED PROTEOGLYCANS J. Biol. Chem., June 30, 2006; 281(26): 17789 - 17800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Sheng, G. Wang, D. P. La Pierre, J. Wen, Z. Deng, C.-K. A. Wong, D. Y. Lee, and B. B. Yang Versican Mediates Mesenchymal-Epithelial Transition Mol. Biol. Cell, April 1, 2006; 17(4): 2009 - 2020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yamagata and J. R. Sanes Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation J. Neurosci., September 14, 2005; 25(37): 8457 - 8467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. K.W. Wong, P. Chiu, S. S.M. Chung, L. M.C. Chow, Y.-Z. Zhao, B. B. Yang, and B. C.B. Ko Pseudolaric Acid B, a Novel Microtubule-Destabilizing Agent That Circumvents Multidrug Resistance Phenotype and Exhibits Antitumor Activity In vivo Clin. Cancer Res., August 15, 2005; 11(16): 6002 - 6011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Sheng, G. Wang, Y. Wang, J. Liang, J. Wen, P.-S. Zheng, Y. Wu, V. Lee, J. Slingerland, D. Dumont, et al. The Roles of Versican V1 and V2 Isoforms in Cell Proliferation and Apoptosis Mol. Biol. Cell, March 1, 2005; 16(3): 1330 - 1340. [Abstract] [Full Text] [PDF]
|
|
