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A more recent version of this article appeared on June 1, 2004
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Submitted on September 24, 2003
Revised on March 2, 2004
Accepted on March 17, 2004
1 Wales Heart Research Institute, Department of Cardiology, University of Wales College of Medicine, Cardiff, UK CF14 4XN
2 National Heart and Lung Institute, Imperial College of Science Technology and Medicine, Dovehouse Street, London, UK SW3 6LY
* Corresponding author. E-mail address: georgech{at}cf.ac.uk.
Ryanodine receptors (RyR) function as Ca2+ channels that regulate Ca2+ release from intracellular stores to control a diverse array of cellular processes. The massive cytoplasmic domain of RyR is believed to be responsible for regulating channel function. We investigated interaction between the transmembrane Ca2+ releasing pore and a panel of cytoplasmic domains of the human cardiac RyR in living cells. Expression of eGFP-tagged RyR constructs encoding distinct transmembrane topological models profoundly altered intracellular Ca2+ handling, and was refractory to modulation by ryanodine, FKBP12.6 and caffeine. The impact of coexpressing dsRed-tagged cytoplasmic domains of RyR2 on intracellular Ca2+ phenotype was assessed using confocal microscopy coupled with parallel determination of in situ protein:protein interaction using fluorescence resonance energy transfer (FRET). Dynamic interactions between RyR cytoplasmic and transmembrane domains were mediated by amino acids 3722-4610 (Interacting or ’I’-domain) which critically modulated intracellular Ca2+ handling and restored RyR sensitivity to caffeine activation. These results provide compelling evidence that specific interaction between cytoplasmic and transmembrane domains is an important mechanism in the intrinsic modulation of RyR Ca2+ release channels.
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